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Assay Detail

CHEMBL902403

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Activity at human recombinant S1P5 receptor expressed in CHO cells assessed as increase in calcium release by FLIPR assay
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type CHO
Confidence 9 — Direct single protein target
Curated By Intermediate

Target

Sphingosine 1-phosphate receptor 5 (CHEMBL2274)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Constrained azacyclic analogues of the immunomodulatory agent FTY720 as molecular probes for sphingosine 1-phosphate receptors.
Hanessian S, Charron G, Billich A, Guerini D.
Bioorg Med Chem Lett (2007) 17 : 491 -494
PubMed 17070046 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 6 8.06 9.04

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL225155 1 9.04
CHEMBL190006 1 8.54
CHEMBL247699 1 8.24
CHEMBL393055 1 7.99
CHEMBL247901 1 7.67
CHEMBL247698 1 6.87

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL225155 EC50 = 0.91 nM 9.04
CHEMBL190006 EC50 = 2.9 nM 8.54
CHEMBL247699 EC50 = 5.8 nM 8.24
CHEMBL393055 EC50 = 10.2 nM 7.99
CHEMBL247901 EC50 = 21.3 nM 7.67
CHEMBL247698 EC50 = 133.8 nM 6.87