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Assay Detail

CHEMBL904994

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]U-69593 from kappa opioid receptor in Hartley guinea pig cerebellum P2 synaptosome membrane
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Cavia porcellus
Tissue Cerebellum
Confidence 9 — Direct single protein target
Curated By Expert

Target

Kappa-type opioid receptor (CHEMBL3952)
Type SINGLE PROTEIN
Organism Cavia porcellus

Publication

Bifunctional [2',6'-dimethyl-L-tyrosine1]endomorphin-2 analogues substituted at position 3 with alkylated phenylalanine derivatives yield potent mixed mu-agonist/delta-antagonist and dual mu-agonist/delta-agonist opioid ligands.
Li T, Shiotani K, Miyazaki A, Tsuda Y, Ambo A, Sasaki Y, Jinsmaa Y, Marczak E, Bryant SD, Lazarus LH, Okada Y.
J Med Chem (2007) 50 : 2753 -2766
PubMed 17497839 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 16 6.52 7.03

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL228409 1 7.03
CHEMBL228503 1 6.76
CHEMBL389948 1 6.75
CHEMBL228516 1 6.69
CHEMBL374818 1 6.56
CHEMBL331325 1 6.31
CHEMBL389949 1 6.08
CHEMBL388800 1 6.00
CHEMBL333357 ENDOMORPHIN 2 1 -
CHEMBL434559 1 -
CHEMBL375274 1 -
CHEMBL389023 1 -
CHEMBL228512 1 -
CHEMBL266122 1 -
CHEMBL413892 1 -
CHEMBL413676 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL228409 Ki = 94.2 nM 7.03
CHEMBL228503 Ki = 172.0 nM 6.76
CHEMBL389948 Ki = 176.0 nM 6.75
CHEMBL228516 Ki = 205.0 nM 6.69
CHEMBL374818 Ki = 278.0 nM 6.56
CHEMBL331325 Ki = 489.0 nM 6.31
CHEMBL389949 Ki = 830.0 nM 6.08
CHEMBL388800 Ki = 998.0 nM 6.00
CHEMBL333357 ENDOMORPHIN 2 Ki > 4000.0 nM -
CHEMBL434559 Ki - -
CHEMBL375274 Ki - -
CHEMBL389023 Ki - -
CHEMBL228512 Ki - -
CHEMBL266122 Ki > 4000.0 nM -
CHEMBL413892 Ki - -
CHEMBL413676 Ki - -