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Assay Detail

CHEMBL904996

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Functional
Agonist activity at mu opioid receptor in Hartley guinea pig ileum assessed as inhibition of electrically-stimulated muscle contraction
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Cavia porcellus
Tissue Ileum
Confidence 9 — Direct single protein target
Curated By Expert

Target

Mu-type opioid receptor (CHEMBL4354)
Type SINGLE PROTEIN
Organism Cavia porcellus

Publication

Bifunctional [2',6'-dimethyl-L-tyrosine1]endomorphin-2 analogues substituted at position 3 with alkylated phenylalanine derivatives yield potent mixed mu-agonist/delta-antagonist and dual mu-agonist/delta-agonist opioid ligands.
Li T, Shiotani K, Miyazaki A, Tsuda Y, Ambo A, Sasaki Y, Jinsmaa Y, Marczak E, Bryant SD, Lazarus LH, Okada Y.
J Med Chem (2007) 50 : 2753 -2766
PubMed 17497839 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 16 8.75 9.92

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL228409 1 9.92
CHEMBL389948 1 9.80
CHEMBL374818 1 9.77
CHEMBL228516 1 9.70
CHEMBL228503 1 9.68
CHEMBL331325 1 9.59
CHEMBL266122 1 9.42
CHEMBL413676 1 8.88
CHEMBL389023 1 8.72
CHEMBL388800 1 8.71
CHEMBL375274 1 8.63
CHEMBL434559 1 8.56
CHEMBL333357 ENDOMORPHIN 2 1 8.16
CHEMBL389949 1 7.84
CHEMBL413892 1 6.41
CHEMBL228512 1 6.27

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL228409 IC50 = 0.12 nM 9.92
CHEMBL389948 IC50 = 0.16 nM 9.80
CHEMBL374818 IC50 = 0.17 nM 9.77
CHEMBL228516 IC50 = 0.2 nM 9.70
CHEMBL228503 IC50 = 0.21 nM 9.68
CHEMBL331325 IC50 = 0.26 nM 9.59
CHEMBL266122 IC50 = 0.38 nM 9.42
CHEMBL413676 IC50 = 1.33 nM 8.88
CHEMBL389023 IC50 = 1.9 nM 8.72
CHEMBL388800 IC50 = 1.94 nM 8.71
CHEMBL375274 IC50 = 2.35 nM 8.63
CHEMBL434559 IC50 = 2.74 nM 8.56
CHEMBL333357 ENDOMORPHIN 2 IC50 = 6.9 nM 8.16
CHEMBL389949 IC50 = 14.4 nM 7.84
CHEMBL413892 IC50 = 389.0 nM 6.41
CHEMBL228512 IC50 = 541.0 nM 6.27