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Assay Detail

CHEMBL913121

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of mouse recombinant inducible NOS by hemoglobin capture assay
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Mus musculus
Confidence 9 — Direct single protein target
Curated By Intermediate

Target

Nitric oxide synthase, inducible (CHEMBL3464)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Structure-based design and synthesis of N(omega)-nitro-L-arginine-containing peptidomimetics as selective inhibitors of neuronal nitric oxide synthase. Displacement of the heme structural water.
Seo J, Igarashi J, Li H, Martasek P, Roman LJ, Poulos TL, Silverman RB.
J Med Chem (2007) 50 : 2089 -2099
PubMed 17425297 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 6 4.82 5.37

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL227744 NITROARGININE 1.0 1 5.37
CHEMBL427286 1 4.80
CHEMBL375949 1 4.80
CHEMBL228077 1 4.77
CHEMBL227937 1 4.75
CHEMBL228212 1 4.43

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL227744 NITROARGININE Ki = 4280.0 nM 5.37
CHEMBL427286 Ki = 16000.0 nM 4.80
CHEMBL375949 Ki = 16000.0 nM 4.80
CHEMBL228077 Ki = 17000.0 nM 4.77
CHEMBL227937 Ki = 18000.0 nM 4.75
CHEMBL228212 Ki = 37000.0 nM 4.43