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Assay Detail

CHEMBL915779

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Induction of human HL60 cell differentiation to monocytes by NBT reduction assay
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HL-60
Confidence 1 — Organism
Curated By Autocuration

Target

HL-60 (CHEMBL383)
Type CELL-LINE
Organism Homo sapiens

Publication

22-Alkyl-20-epi-1alpha,25-dihydroxyvitamin D3 compounds of superagonistic activity: syntheses, biological activities and interaction with the receptor.
Yamamoto K, Inaba Y, Yoshimoto N, Choi M, DeLuca HF, Yamada S.
J Med Chem (2007) 50 : 932 -939
PubMed 17298045 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 4 9.66 10.74

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL220846 1 10.74
CHEMBL222195 1 10.24
CHEMBL375811 1 8.98
CHEMBL846 CALCITRIOL 4.0 1 8.67

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL220846 EC50 = 0.018 nM 10.74
CHEMBL222195 EC50 = 0.057 nM 10.24
CHEMBL375811 EC50 = 1.05 nM 8.98
CHEMBL846 CALCITRIOL EC50 = 2.15 nM 8.67