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Assay Detail

CHEMBL917731

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [125]NDP-MSH from human MC4 receptor expressed in HEK293 cells
15
Total Activities
14
Compounds Tested
3
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Expert

Target

Melanocortin receptor 4 (CHEMBL259)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

4-{(2R)-[3-Aminopropionylamido]-3-(2,4-dichlorophenyl)propionyl}-1-{2-[(2-thienyl)ethylaminomethyl]phenyl}piperazine as a potent and selective melanocortin-4 receptor antagonist--design, synthesis, and characterization.
Chen C, Pontillo J, Fleck BA, Gao Y, Wen J, Tran JA, Tucci FC, Marinkovic D, Foster AC, Saunders J.
J Med Chem (2004) 47 : 6821 -6830
PubMed 15615531 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 10 7.70 8.92
IC50 4 8.01 9.52
Activity 1 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL140154 1 9.52
CHEMBL339053 2 8.92
CHEMBL140738 1 8.92
CHEMBL365597 1 8.74
CHEMBL360716 1 8.19
CHEMBL366706 1 7.97
CHEMBL186083 1 7.69
CHEMBL390130 1 7.36
CHEMBL186841 1 7.16
CHEMBL140155 1 6.97
CHEMBL143131 1 6.62
CHEMBL340355 1 6.57
CHEMBL183434 1 6.42
CHEMBL424661 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL140154 IC50 = 0.3 nM 9.52
CHEMBL339053 IC50 = 1.2 nM 8.92
CHEMBL140738 Ki = 1.2 nM 8.92
CHEMBL365597 Ki = 1.8 nM 8.74
CHEMBL360716 Ki = 6.4 nM 8.19
CHEMBL339053 Ki = 9.8 nM 8.01
CHEMBL366706 Ki = 10.8 nM 7.97
CHEMBL186083 Ki = 20.5 nM 7.69
CHEMBL390130 Ki = 44.0 nM 7.36
CHEMBL186841 Ki = 69.5 nM 7.16
CHEMBL140155 IC50 = 108.0 nM 6.97
CHEMBL143131 IC50 = 238.0 nM 6.62
CHEMBL340355 Ki = 270.0 nM 6.57
CHEMBL183434 Ki = 380.0 nM 6.42
CHEMBL424661 Activity - -