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Assay Detail

CHEMBL917733

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antagonist activity at human MC4 receptor expressed in HEK293 cells assessed as inhibition of alpha-MSH-stimulated cAMP production
11
Total Activities
10
Compounds Tested
3
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Expert

Target

Melanocortin receptor 4 (CHEMBL259)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

4-{(2R)-[3-Aminopropionylamido]-3-(2,4-dichlorophenyl)propionyl}-1-{2-[(2-thienyl)ethylaminomethyl]phenyl}piperazine as a potent and selective melanocortin-4 receptor antagonist--design, synthesis, and characterization.
Chen C, Pontillo J, Fleck BA, Gao Y, Wen J, Tran JA, Tucci FC, Marinkovic D, Foster AC, Saunders J.
J Med Chem (2004) 47 : 6821 -6830
PubMed 15615531 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Intrinsic activity 9 - -
Kd 1 7.90 7.90
Activity 1 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL365597 2 7.90
CHEMBL140155 1 -
CHEMBL339053 1 -
CHEMBL340355 1 -
CHEMBL183434 1 -
CHEMBL366706 1 -
CHEMBL360716 1 -
CHEMBL390130 1 -
CHEMBL186083 1 -
CHEMBL186841 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL365597 Kd = 12.59 nM 7.90
CHEMBL365597 Intrinsic activity = 3.0 % -
CHEMBL140155 Activity - -
CHEMBL339053 Intrinsic activity = 100.0 % -
CHEMBL340355 Intrinsic activity = 105.0 % -
CHEMBL183434 Intrinsic activity = 131.0 % -
CHEMBL366706 Intrinsic activity = 105.0 % -
CHEMBL360716 Intrinsic activity = 100.0 % -
CHEMBL390130 Intrinsic activity = 102.0 % -
CHEMBL186083 Intrinsic activity = 14.0 % -
CHEMBL186841 Intrinsic activity = 16.0 % -