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Assay Detail

CHEMBL919632

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of RFC-mediated [3H]MTX influx into human CCRF-CEM cells
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type CCRF-CEM
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Reduced folate transporter (CHEMBL4833)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis and in vitro antifolate activity of rotationally restricted aminopterin and methotrexate analogues.
Rosowsky A, Forsch RA, Wright JE.
J Med Chem (2004) 47 : 6958 -6963
PubMed 15615544 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 3 6.26 6.55

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL390990 1 6.55
CHEMBL388501 1 6.28
CHEMBL224946 1 5.96

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL390990 Ki = 280.0 nM 6.55
CHEMBL388501 Ki = 530.0 nM 6.28
CHEMBL224946 Ki = 1100.0 nM 5.96