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Assay Detail

CHEMBL924283

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of SAENTA-fluorescein from human ENT1 in K562 cells after 45 mins by flow cytometry
22
Total Activities
22
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type K562
Confidence 9 — Direct single protein target
Curated By Intermediate

Target

Equilibrative nucleoside transporter 1 (CHEMBL1997)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Novel C2-purine position analogs of nitrobenzylmercaptopurine riboside as human equilibrative nucleoside transporter 1 inhibitors.
Gupte A, Buolamwini JK.
Bioorg Med Chem (2007) 15 : 7726 -7737
PubMed 17881236 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 22 7.32 9.15

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL418509 NITROBENZYLMERCAPTOPURINE RIBONUCLEOSIDE 1 9.15
CHEMBL394171 1 8.96
CHEMBL239246 1 8.68
CHEMBL393342 1 7.96
CHEMBL239889 1 7.96
CHEMBL239670 1 7.96
CHEMBL391730 1 7.62
CHEMBL394012 1 7.62
CHEMBL391838 1 7.60
CHEMBL239454 1 7.48
CHEMBL240101 1 7.47
CHEMBL239887 1 7.07
CHEMBL393322 1 6.90
CHEMBL239248 1 6.71
CHEMBL391651 1 6.61
CHEMBL239888 1 6.61
CHEMBL240102 1 6.50
CHEMBL393120 1 6.49
CHEMBL239455 1 6.45
CHEMBL428031 1 6.41
CHEMBL239669 1 6.40
CHEMBL239451 1 6.37

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL418509 NITROBENZYLMERCAPTOPURINE RIBONUCLEOSIDE Ki = 0.7 nM 9.15
CHEMBL394171 Ki = 1.1 nM 8.96
CHEMBL239246 Ki = 2.1 nM 8.68
CHEMBL393342 Ki = 11.0 nM 7.96
CHEMBL239889 Ki = 11.0 nM 7.96
CHEMBL239670 Ki = 11.0 nM 7.96
CHEMBL391730 Ki = 24.0 nM 7.62
CHEMBL394012 Ki = 24.0 nM 7.62
CHEMBL391838 Ki = 25.0 nM 7.60
CHEMBL239454 Ki = 33.0 nM 7.48
CHEMBL240101 Ki = 34.0 nM 7.47
CHEMBL239887 Ki = 86.0 nM 7.07
CHEMBL393322 Ki = 125.0 nM 6.90
CHEMBL239248 Ki = 193.0 nM 6.71
CHEMBL391651 Ki = 247.0 nM 6.61
CHEMBL239888 Ki = 243.0 nM 6.61
CHEMBL240102 Ki = 315.0 nM 6.50
CHEMBL393120 Ki = 325.0 nM 6.49
CHEMBL239455 Ki = 357.0 nM 6.45
CHEMBL428031 Ki = 394.0 nM 6.41
CHEMBL239669 Ki = 400.0 nM 6.40
CHEMBL239451 Ki = 429.0 nM 6.37