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Assay Detail

CHEMBL925469

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Agonist activity at ERalpha expressed in HEK293 cells by transactivation assay
34
Total Activities
17
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Intermediate

Target

Estrogen receptor (CHEMBL206)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Androstene-3,5-dienes as ER-beta selective SERMs.
Blizzard TA, Gude C, Morgan JD, Chan W, Birzin ET, Mojena M, Tudela C, Chen F, Knecht K, Su Q, Kraker B, Mosley RT, Holmes MA, Rohrer SP, Hammond ML.
Bioorg Med Chem Lett (2007) 17 : 6295 -6298
PubMed 17890084 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 17 6.83 9.12
Activity 17 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL135 ESTRADIOL 4.0 2 9.12
CHEMBL440283 ANDROSTENEDIOL -1.0 2 7.58
CHEMBL398841 2 6.89
CHEMBL400480 2 6.37
CHEMBL249904 2 6.31
CHEMBL250110 2 6.27
CHEMBL430395 2 6.09
CHEMBL246138 2 6.01
CHEMBL246140 2 -
CHEMBL249700 2 -
CHEMBL250109 2 -
CHEMBL398634 2 -
CHEMBL398840 2 -
CHEMBL249903 2 -
CHEMBL398456 2 -
CHEMBL251515 2 -
CHEMBL398455 2 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL135 ESTRADIOL EC50 = 0.75 nM 9.12
CHEMBL440283 ANDROSTENEDIOL EC50 = 26.0 nM 7.58
CHEMBL398841 EC50 = 130.0 nM 6.89
CHEMBL400480 EC50 = 430.0 nM 6.37
CHEMBL249904 EC50 = 490.0 nM 6.31
CHEMBL250110 EC50 = 540.0 nM 6.27
CHEMBL430395 EC50 = 820.0 nM 6.09
CHEMBL246138 EC50 = 980.0 nM 6.01
CHEMBL398634 EC50 > 1000.0 nM -
CHEMBL246138 Activity = 35.0 % -
CHEMBL249904 Activity = 15.0 % -
CHEMBL250109 EC50 > 1000.0 nM -
CHEMBL398841 Activity = 82.0 % -
CHEMBL440283 ANDROSTENEDIOL Activity = 91.0 % -
CHEMBL400480 Activity = 49.0 % -
CHEMBL249700 Activity = 29.0 % -
CHEMBL249700 EC50 > 1000.0 nM -
CHEMBL250110 Activity = 49.0 % -
CHEMBL246140 Activity = 12.0 % -
CHEMBL246140 EC50 > 1000.0 nM -
CHEMBL135 ESTRADIOL Activity = 100.0 % -
CHEMBL398455 Activity = 28.0 % -
CHEMBL398455 EC50 > 1000.0 nM -
CHEMBL251515 Activity = 27.0 % -
CHEMBL251515 EC50 > 1000.0 nM -
CHEMBL398456 Activity = 4.0 % -
CHEMBL398456 EC50 > 1000.0 nM -
CHEMBL249903 Activity = 3.0 % -
CHEMBL249903 EC50 > 1000.0 nM -
CHEMBL398840 Activity = - % -
CHEMBL398840 EC50 > 1000.0 nM -
CHEMBL430395 Activity = 38.0 % -
CHEMBL250109 Activity = 13.0 % -
CHEMBL398634 Activity = 4.0 % -