Assay Detail
Binding
CHEMBL929019
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Displacement of [3H]NMS from human cloned muscarinic M4 receptor expressed in CHO cells
8
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | CHO |
| Confidence | 9 — Direct single protein target |
| Curated By | Intermediate |
Target
Muscarinic acetylcholine receptor M4 (CHEMBL1821) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Rapid novel divergent synthesis and muscarinic agonist profile of all four optical isomers of N,N,N-trimethyl(6-methyl-1,4-dioxan-2-yl)methanaminium iodide.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Ki | 8 | 4.68 | 5.20 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL14 | CARBACHOL | 4.0 | 1 | 5.20 |
| CHEMBL667 | ACETYLCHOLINE | 4.0 | 1 | 5.07 |
| CHEMBL390842 | — | — | 1 | 5.01 |
| CHEMBL228144 | — | — | 2 | 4.94 |
| CHEMBL227429 | — | — | 1 | 4.34 |
| CHEMBL227378 | — | — | 1 | 4.16 |
| CHEMBL404557 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL14 | CARBACHOL | Ki | = | 6309.57 | nM | 5.20 |
| CHEMBL667 | ACETYLCHOLINE | Ki | = | 8511.38 | nM | 5.07 |
| CHEMBL390842 | — | Ki | = | 9772.37 | nM | 5.01 |
| CHEMBL228144 | — | Ki | = | 11481.54 | nM | 4.94 |
| CHEMBL227429 | — | Ki | = | 45708.82 | nM | 4.34 |
| CHEMBL227378 | — | Ki | = | 69183.1 | nM | 4.16 |
| CHEMBL228144 | — | Ki | = | 87096.36 | nM | 4.06 |
| CHEMBL404557 | — | Ki | < | 100000.0 | nM | - |