Assay Detail
Functional
CHEMBL929415
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Antagonist activity at human muscarinic M4 receptor expressed in CHO cells assessed as blockade of carbachol-induced inhibition of forskolin-stimulated cAMP accumulation
5
Total Activities
3
Compounds Tested
2
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Functional |
| Organism | Homo sapiens |
| Cell Type | CHO |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Muscarinic acetylcholine receptor M4 (CHEMBL1821) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Diphenidol-related diamines as novel muscarinic M4 receptor antagonists.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 3 | 7.10 | 7.27 |
| IC50 | 2 | - | - |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL59898 | — | — | 2 | 7.27 |
| CHEMBL402207 | — | — | 2 | 7.17 |
| CHEMBL403773 | — | — | 1 | 6.86 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL59898 | — | EC50 | = | 54.0 | nM | 7.27 |
| CHEMBL402207 | — | EC50 | = | 67.0 | nM | 7.17 |
| CHEMBL403773 | — | EC50 | = | 138.0 | nM | 6.86 |
| CHEMBL59898 | — | IC50 | > | 1000.0 | nM | - |
| CHEMBL402207 | — | IC50 | > | 1000.0 | nM | - |