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Assay Detail

CHEMBL931203

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Effect on Wnt/beta-casein reporter pathway in HEK293 cells expressing super(8X)TOPFlash reporter assessed as luciferase activity in presence of Wnt-3a
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HEK293
Confidence 1 — Organism
Curated By Autocuration

Target

HEK293 (CHEMBL614818)
Type CELL-LINE
Organism Homo sapiens

Publication

Small-molecule synergist of the Wnt/beta-catenin signaling pathway.
Zhang Q, Major MB, Takanashi S, Camp ND, Nishiya N, Peters EC, Ginsberg MH, Jian X, Randazzo PA, Schultz PG, Moon RT, Ding S.
Proc Natl Acad Sci U S A (2007) 104 : 7444 -7448
PubMed 17460038 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 12 5.91 6.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL404773 1 6.40
CHEMBL259181 1 6.30
CHEMBL265685 1 6.22
CHEMBL260162 1 5.82
CHEMBL261879 1 5.77
CHEMBL406603 1 5.51
CHEMBL405112 1 5.34
CHEMBL259182 1 -
CHEMBL428131 1 -
CHEMBL261353 1 -
CHEMBL407988 1 -
CHEMBL263110 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL404773 EC50 = 400.0 nM 6.40
CHEMBL259181 EC50 = 500.0 nM 6.30
CHEMBL265685 EC50 = 600.0 nM 6.22
CHEMBL260162 EC50 = 1500.0 nM 5.82
CHEMBL261879 EC50 = 1700.0 nM 5.77
CHEMBL406603 EC50 = 3100.0 nM 5.51
CHEMBL405112 EC50 = 4600.0 nM 5.34
CHEMBL259182 EC50 > 7100.0 nM -
CHEMBL428131 EC50 > 7100.0 nM -
CHEMBL261353 EC50 > 7100.0 nM -
CHEMBL407988 EC50 > 7100.0 nM -
CHEMBL263110 EC50 > 7100.0 nM -