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Assay Detail

CHEMBL931250

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Functional
Antagonist activity against human EP1 receptor expressed in CHOK1 cells assessed as inhibition of PGE2-induced intracellular calcium mobilization by FLIPR assay
10
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type CHO-K1
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Prostaglandin E2 receptor EP1 subtype (CHEMBL1811)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of a novel indole series of EP1 receptor antagonists by scaffold hopping.
Hall A, Billinton A, Brown SH, Chowdhury A, Giblin GM, Goldsmith P, Hurst DN, Naylor A, Patel S, Scoccitti T, Theobald PJ.
Bioorg Med Chem Lett (2008) 18 : 2684 -2690
PubMed 18378447 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 10 8.76 10.20

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL402392 1 10.20
CHEMBL257134 1 9.70
CHEMBL257997 2 9.30
CHEMBL402338 1 9.00
CHEMBL403407 1 8.90
CHEMBL255675 1 8.40
CHEMBL401785 1 7.90
CHEMBL254763 1 7.70
CHEMBL436656 1 7.20

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL402392 Ki = 0.0631 nM 10.20
CHEMBL257134 Ki = 0.1995 nM 9.70
CHEMBL257997 Ki = 0.5012 nM 9.30
CHEMBL257997 Ki = 0.5 nM 9.30
CHEMBL402338 Ki = 1.0 nM 9.00
CHEMBL403407 Ki = 1.259 nM 8.90
CHEMBL255675 Ki = 3.981 nM 8.40
CHEMBL401785 Ki = 12.59 nM 7.90
CHEMBL254763 Ki = 19.95 nM 7.70
CHEMBL436656 Ki = 63.1 nM 7.20