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Assay Detail

CHEMBL931441

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of hypoxia-induced HIF1 activation in human AGS cells by reporter gene assay
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type AGS
Confidence 5 — Multiple protein complex / RNA
Curated By Autocuration

Target

Hypoxia inducible factors; HIF-1-alpha, HIF-2-alpha (CHEMBL2221345)
Type PROTEIN FAMILY
Organism Homo sapiens

Publication

Hypoxia-inducible factor-1 and nuclear factor-kappaB inhibitory meroterpene analogues of bakuchiol, a constituent of the seeds of Psoralea corylifolia.
Wu CZ, Hong SS, Cai XF, Dat NT, Nan JX, Hwang BY, Lee JJ, Lee D.
Bioorg Med Chem Lett (2008) 18 : 2619 -2623
PubMed 18359631 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 8 5.71 8.44

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL383824 ALVESPIMYCIN 2.0 1 8.44
CHEMBL408851 ACETYLBAKUCHIOL 1 5.24
CHEMBL262344 BAKUCHIOL 1.0 1 5.21
CHEMBL262630 1 5.06
CHEMBL260776 1 4.58
CHEMBL261315 1 -
CHEMBL405046 1 -
CHEMBL258478 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL383824 ALVESPIMYCIN IC50 = 3.6 nM 8.44
CHEMBL408851 ACETYLBAKUCHIOL IC50 = 5700.0 nM 5.24
CHEMBL262344 BAKUCHIOL IC50 = 6100.0 nM 5.21
CHEMBL262630 IC50 = 8700.0 nM 5.06
CHEMBL260776 IC50 = 26300.0 nM 4.58
CHEMBL261315 IC50 > 50000.0 nM -
CHEMBL405046 IC50 > 50000.0 nM -
CHEMBL258478 IC50 > 50000.0 nM -