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Assay Detail

CHEMBL934255

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antagonist activity at human PR expressed in human T47D cells assessed as inhibition of progesterone induced alkaline phosphatase
25
Total Activities
25
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type T47D
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Progesterone receptor (CHEMBL208)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design, synthesis, and SAR of new pyrrole-oxindole progesterone receptor modulators leading to 5-(7-fluoro-3,3-dimethyl-2-oxo-2,3-dihydro-1H-indol-5-yl)-1-methyl-1H-pyrrole-2-carbonitrile (WAY-255348).
Fensome A, Adams WR, Adams AL, Berrodin TJ, Cohen J, Huselton C, Illenberger A, Kern JC, Hudak VA, Marella MA, Melenski EG, McComas CC, Mugford CA, Slayden OD, Yudt M, Zhang Z, Zhang P, Zhu Y, Winneker RC, Wrobel JE.
J Med Chem (2008) 51 : 1861 -1873
PubMed 18318463 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 25 7.74 9.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1276308 MIFEPRISTONE 4.0 1 9.70
CHEMBL407847 1 8.49
CHEMBL272663 1 8.48
CHEMBL429572 1 8.35
CHEMBL429928 1 8.31
CHEMBL407848 1 8.30
CHEMBL270714 1 8.23
CHEMBL270908 1 8.00
CHEMBL271804 1 7.89
CHEMBL271552 1 7.82
CHEMBL308938 1 7.82
CHEMBL270052 1 7.70
CHEMBL406235 1 7.69
CHEMBL271149 1 7.60
CHEMBL117187 1 7.57
CHEMBL437801 1 7.57
CHEMBL405428 1 7.54
CHEMBL271175 1 7.52
CHEMBL437622 1 7.52
CHEMBL407622 1 7.52
CHEMBL271336 1 7.32
CHEMBL271835 1 6.79
CHEMBL408696 1 6.71
CHEMBL270909 1 6.52
CHEMBL409147 1 6.46

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1276308 MIFEPRISTONE IC50 = 0.2 nM 9.70
CHEMBL407847 IC50 = 3.2 nM 8.49
CHEMBL272663 IC50 = 3.3 nM 8.48
CHEMBL429572 IC50 = 4.5 nM 8.35
CHEMBL429928 IC50 = 4.9 nM 8.31
CHEMBL407848 IC50 = 5.0 nM 8.30
CHEMBL270714 IC50 = 5.9 nM 8.23
CHEMBL270908 IC50 = 10.0 nM 8.00
CHEMBL271804 IC50 = 13.0 nM 7.89
CHEMBL271552 IC50 = 15.0 nM 7.82
CHEMBL308938 IC50 = 15.0 nM 7.82
CHEMBL270052 IC50 = 20.0 nM 7.70
CHEMBL406235 IC50 = 20.3 nM 7.69
CHEMBL271149 IC50 = 25.0 nM 7.60
CHEMBL117187 IC50 = 27.0 nM 7.57
CHEMBL437801 IC50 = 27.0 nM 7.57
CHEMBL405428 IC50 = 29.0 nM 7.54
CHEMBL271175 IC50 = 30.0 nM 7.52
CHEMBL437622 IC50 = 30.0 nM 7.52
CHEMBL407622 IC50 = 30.0 nM 7.52
CHEMBL271336 IC50 = 48.0 nM 7.32
CHEMBL271835 IC50 = 164.0 nM 6.79
CHEMBL408696 IC50 = 194.0 nM 6.71
CHEMBL270909 IC50 = 300.0 nM 6.52
CHEMBL409147 IC50 = 351.0 nM 6.46