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Assay Detail

CHEMBL934433

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human recombinant Sirt2 expressed in Escherichia coli BL21 by fluorescent deacetylase assay
23
Total Activities
23
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

NAD-dependent protein deacetylase sirtuin-2 (CHEMBL4462)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Structure-activity studies on splitomicin derivatives as sirtuin inhibitors and computational prediction of binding mode.
Neugebauer RC, Uchiechowska U, Meier R, Hruby H, Valkov V, Verdin E, Sippl W, Jung M.
J Med Chem (2008) 51 : 1203 -1213
PubMed 18269226 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 23 5.25 6.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL270110 1 6.00
CHEMBL438203 1 5.82
CHEMBL407321 1 5.82
CHEMBL273076 1 5.70
CHEMBL407517 1 5.64
CHEMBL270478 1 5.64
CHEMBL407059 1 5.51
CHEMBL409006 1 5.50
CHEMBL270109 1 5.47
CHEMBL261605 1 5.47
CHEMBL271536 1 5.36
CHEMBL270292 1 5.28
CHEMBL270291 1 5.27
CHEMBL402446 1 5.19
CHEMBL405156 1 5.10
CHEMBL429433 1 5.01
CHEMBL270075 1 5.00
CHEMBL403988 1 4.97
CHEMBL270905 1 4.71
CHEMBL272461 1 4.71
CHEMBL406803 1 4.70
CHEMBL1140 NIACINAMIDE 4.0 1 4.49
CHEMBL380797 SIRTINOL 1 4.28

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL270110 IC50 = 1000.0 nM 6.00
CHEMBL438203 IC50 = 1500.0 nM 5.82
CHEMBL407321 IC50 = 1500.0 nM 5.82
CHEMBL273076 IC50 = 2000.0 nM 5.70
CHEMBL407517 IC50 = 2300.0 nM 5.64
CHEMBL270478 IC50 = 2300.0 nM 5.64
CHEMBL407059 IC50 = 3100.0 nM 5.51
CHEMBL409006 IC50 = 3200.0 nM 5.50
CHEMBL270109 IC50 = 3400.0 nM 5.47
CHEMBL261605 IC50 = 3400.0 nM 5.47
CHEMBL271536 IC50 = 4400.0 nM 5.36
CHEMBL270292 IC50 = 5200.0 nM 5.28
CHEMBL270291 IC50 = 5400.0 nM 5.27
CHEMBL402446 IC50 = 6400.0 nM 5.19
CHEMBL405156 IC50 = 7900.0 nM 5.10
CHEMBL429433 IC50 = 9800.0 nM 5.01
CHEMBL270075 IC50 = 10100.0 nM 5.00
CHEMBL403988 IC50 = 10600.0 nM 4.97
CHEMBL270905 IC50 = 19500.0 nM 4.71
CHEMBL272461 IC50 = 19700.0 nM 4.71
CHEMBL406803 IC50 = 20000.0 nM 4.70
CHEMBL1140 NIACINAMIDE IC50 = 32300.0 nM 4.49
CHEMBL380797 SIRTINOL IC50 = 53000.0 nM 4.28