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Assay Detail

CHEMBL940955

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Functional
Inhibition of KSP-induced nucleolin phosphorylation in cell-based mitotic arrest assay
15
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Confidence 8 — Homologous single protein target
Curated By Intermediate

Target

Kinesin-like protein KIF11 (CHEMBL4581)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Kinesin spindle protein (KSP) inhibitors. Part 8: Design and synthesis of 1,4-diaryl-4,5-dihydropyrazoles as potent inhibitors of the mitotic kinesin KSP.
Roecker AJ, Coleman PJ, Mercer SP, Schreier JD, Buser CA, Walsh ES, Hamilton K, Lobell RB, Tao W, Diehl RE, South VJ, Davide JP, Kohl NE, Yan Y, Kuo LC, Li C, Fernandez-Metzler C, Mahan EA, Prueksaritanont T, Hartman GD.
Bioorg Med Chem Lett (2007) 17 : 5677 -5682
PubMed 17766111 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 15 7.40 8.49

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL247044 1 8.49
CHEMBL250125 1 8.30
CHEMBL246604 1 8.03
CHEMBL246603 1 7.96
CHEMBL246422 1 7.82
CHEMBL443490 1 7.57
CHEMBL392599 1 7.42
CHEMBL248244 1 7.40
CHEMBL247414 1 7.34
CHEMBL246001 1 7.28
CHEMBL248400 1 7.11
CHEMBL401006 1 6.86
CHEMBL247594 1 6.68
CHEMBL393647 1 6.43
CHEMBL246000 1 6.25

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL247044 EC50 = 3.2 nM 8.49
CHEMBL250125 EC50 = 5.0 nM 8.30
CHEMBL246604 EC50 = 9.4 nM 8.03
CHEMBL246603 EC50 = 11.0 nM 7.96
CHEMBL246422 EC50 = 15.0 nM 7.82
CHEMBL443490 EC50 = 27.0 nM 7.57
CHEMBL392599 EC50 = 38.0 nM 7.42
CHEMBL248244 EC50 = 40.0 nM 7.40
CHEMBL247414 EC50 = 46.0 nM 7.34
CHEMBL246001 EC50 = 53.0 nM 7.28
CHEMBL248400 EC50 = 78.0 nM 7.11
CHEMBL401006 EC50 = 138.0 nM 6.86
CHEMBL247594 EC50 = 208.0 nM 6.68
CHEMBL393647 EC50 = 368.0 nM 6.43
CHEMBL246000 EC50 = 567.0 nM 6.25