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Assay Detail

CHEMBL946893

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of cytokine-induced iNOS activity in human DLD1 cells after 24 hrs
27
Total Activities
27
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type DLD-1
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Nitric oxide synthase, inducible (CHEMBL4481)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of a series of aminopiperidines as novel iNOS inhibitors.
Le Bourdonnec B, Leister LK, Ajello CA, Cassel JA, Seida PR, O'Hare H, Gu M, Chu GH, Tuthill PA, DeHaven RN, Dolle RE.
Bioorg Med Chem Lett (2008) 18 : 336 -343
PubMed 18024030 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 27 5.71 6.68

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL447183 1 6.68
CHEMBL256378 1 6.55
CHEMBL256414 1 6.50
CHEMBL428248 1 6.42
CHEMBL256415 1 6.24
CHEMBL403952 1 6.24
CHEMBL402217 1 6.24
CHEMBL256402 1 6.18
CHEMBL272708 1 5.96
CHEMBL271246 1 5.92
CHEMBL256618 1 5.85
CHEMBL255599 1 5.82
CHEMBL402425 1 5.50
CHEMBL428069 1 5.48
CHEMBL257007 1 5.46
CHEMBL256620 1 5.46
CHEMBL436643 1 5.43
CHEMBL256249 1 5.43
CHEMBL257008 1 5.42
CHEMBL271248 1 5.36
CHEMBL272271 1 5.36
CHEMBL258310 1 5.35
CHEMBL256376 1 5.30
CHEMBL272697 1 5.23
CHEMBL255124 1 5.17
CHEMBL271247 1 5.10
CHEMBL7889 L-NIL 1 4.51

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL447183 IC50 = 210.0 nM 6.68
CHEMBL256378 IC50 = 280.0 nM 6.55
CHEMBL256414 IC50 = 320.0 nM 6.50
CHEMBL428248 IC50 = 380.0 nM 6.42
CHEMBL256415 IC50 = 570.0 nM 6.24
CHEMBL403952 IC50 = 580.0 nM 6.24
CHEMBL402217 IC50 = 570.0 nM 6.24
CHEMBL256402 IC50 = 660.0 nM 6.18
CHEMBL272708 IC50 = 1100.0 nM 5.96
CHEMBL271246 IC50 = 1200.0 nM 5.92
CHEMBL256618 IC50 = 1400.0 nM 5.85
CHEMBL255599 IC50 = 1500.0 nM 5.82
CHEMBL402425 IC50 = 3200.0 nM 5.50
CHEMBL428069 IC50 = 3300.0 nM 5.48
CHEMBL257007 IC50 = 3500.0 nM 5.46
CHEMBL256620 IC50 = 3500.0 nM 5.46
CHEMBL436643 IC50 = 3700.0 nM 5.43
CHEMBL256249 IC50 = 3700.0 nM 5.43
CHEMBL257008 IC50 = 3800.0 nM 5.42
CHEMBL271248 IC50 = 4400.0 nM 5.36
CHEMBL272271 IC50 = 4400.0 nM 5.36
CHEMBL258310 IC50 = 4500.0 nM 5.35
CHEMBL256376 IC50 = 5000.0 nM 5.30
CHEMBL272697 IC50 = 5900.0 nM 5.23
CHEMBL255124 IC50 = 6700.0 nM 5.17
CHEMBL271247 IC50 = 8000.0 nM 5.10
CHEMBL7889 L-NIL IC50 = 31000.0 nM 4.51