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Assay Detail

CHEMBL950400

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antiproliferative activity against human HN5 cells overexpressing EGFR after 3 days by methylene blue staining
17
Total Activities
17
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HN5
Confidence 1 — Organism
Curated By Autocuration

Target

HN5 (CHEMBL614828)
Type CELL-LINE
Organism Homo sapiens

Publication

Dual EGFR/ErbB-2 inhibitors from novel pyrrolidinyl-acetylenic thieno[3,2-d]pyrimidines.
Hubbard RD, Dickerson SH, Emerson HK, Griffin RJ, Reno MJ, Hornberger KR, Rusnak DW, Wood ER, Uehling DE, Waterson AG.
Bioorg Med Chem Lett (2008) 18 : 5738 -5738
PubMed 18842405 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 17 6.80 7.43

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL495734 1 7.43
CHEMBL500381 1 7.28
CHEMBL466586 1 7.24
CHEMBL512076 GSK-192082A 1 7.02
CHEMBL497184 1 7.00
CHEMBL517123 GSK-182497A 1 7.00
CHEMBL525805 1 6.95
CHEMBL501705 1 6.90
CHEMBL507329 GW869810X 1 6.80
CHEMBL497804 1 6.80
CHEMBL523573 1 6.72
CHEMBL500640 1 6.70
CHEMBL496174 1 6.62
CHEMBL500115 1 6.42
CHEMBL495979 1 6.27
CHEMBL495733 1 6.26
CHEMBL497803 1 6.23

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL495734 IC50 = 37.0 nM 7.43
CHEMBL500381 IC50 = 52.0 nM 7.28
CHEMBL466586 IC50 = 58.0 nM 7.24
CHEMBL512076 GSK-192082A IC50 = 95.0 nM 7.02
CHEMBL497184 IC50 = 99.0 nM 7.00
CHEMBL517123 GSK-182497A IC50 = 100.0 nM 7.00
CHEMBL525805 IC50 = 113.0 nM 6.95
CHEMBL501705 IC50 = 127.0 nM 6.90
CHEMBL507329 GW869810X IC50 = 160.0 nM 6.80
CHEMBL497804 IC50 = 160.0 nM 6.80
CHEMBL523573 IC50 = 190.0 nM 6.72
CHEMBL500640 IC50 = 200.0 nM 6.70
CHEMBL496174 IC50 = 238.0 nM 6.62
CHEMBL500115 IC50 = 380.0 nM 6.42
CHEMBL495979 IC50 = 536.0 nM 6.27
CHEMBL495733 IC50 = 550.0 nM 6.26
CHEMBL497803 IC50 = 590.0 nM 6.23