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Assay Detail

CHEMBL951707

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Stimulation of disulfide bond cross-linking formation in rat M3'(3C)-Xa receptor A91C/T549C mutant expressed in african green monkey COS7 cells by scanning densitometry
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Cell Type COS-7
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Muscarinic acetylcholine receptor M3 (CHEMBL320)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Distinct structural changes in a G protein-coupled receptor caused by different classes of agonist ligands.
Li JH, Han SJ, Hamdan FF, Kim SK, Jacobson KA, Bloodworth LM, Zhang X, Wess J.
J Biol Chem (2007) 282 : 26284 -26293
PubMed 17623649 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 1 10.30 10.30

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL376897 METHSCOPOLAMINE 4.0 1 10.30

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL376897 METHSCOPOLAMINE EC50 = 0.05 nM 10.30