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Assay Detail

CHEMBL952530

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Activity at rat wild type GABAA alpha-1-beta-2-gamma-2 receptor expressed in xenopus oocytes assessed as GABA-elicited response by two electrode voltage clamp method relative to control
7
Total Activities
4
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Cell Type Oocyte
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

Gamma-aminobutyric acid receptor subunit alpha-1 (CHEMBL1962)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Proximity-accelerated chemical coupling reaction in the benzodiazepine-binding site of gamma-aminobutyric acid type A receptors: superposition of different allosteric modulators.
Tan KR, Gonthier A, Baur R, Ernst M, Goeldner M, Sigel E.
J Biol Chem (2007) 282 : 26316 -26325
PubMed 17626010 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
max activation 4 - -
EC50 3 7.43 8.96

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL6597 Ro-154513 2 8.96
CHEMBL911 ZOLPIDEM 4.0 2 6.72
CHEMBL485829 2 6.60
CHEMBL407 FLUMAZENIL 4.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL6597 Ro-154513 EC50 = 1.1 nM 8.96
CHEMBL911 ZOLPIDEM EC50 = 191.0 nM 6.72
CHEMBL485829 EC50 = 250.0 nM 6.60
CHEMBL911 ZOLPIDEM max activation = 429.0 % -
CHEMBL485829 max activation = 11.0 % -
CHEMBL6597 Ro-154513 max activation = -15.0 % -
CHEMBL407 FLUMAZENIL max activation = 12.0 % -