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Assay Detail

CHEMBL958789

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human recombinant 17beta-HSD2 expressed in HEK293 cell lysate assessed as conversion of radiolabeled estrone to estradiol
18
Total Activities
18
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

17-beta-hydroxysteroid dehydrogenase type 2 (CHEMBL2789)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of nonsteroidal 17beta-hydroxysteroid dehydrogenase 1 inhibitors by pharmacophore-based screening of virtual compound libraries.
Schuster D, Nashev LG, Kirchmair J, Laggner C, Wolber G, Langer T, Odermatt A.
J Med Chem (2008) 51 : 4188 -4199
PubMed 18533708 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 18 5.37 6.44

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL150 KAEMPFEROL 1.0 1 6.44
CHEMBL50 QUERCETIN 3.0 1 5.81
CHEMBL131921 BIOCHANIN 1 5.00
CHEMBL9352 NARINGENIN 1 4.84
CHEMBL44 GENISTEIN 2.0 1 4.78
CHEMBL194399 ENDOSULFAN 1 -
CHEMBL51483 GOSSYPOL 3.0 1 -
CHEMBL230006 ENOXOLONE 2.0 1 -
CHEMBL8145 DAIDZEIN 2.0 1 -
CHEMBL117 CHRYSIN 1 -
CHEMBL275638 FLAVONE 2.0 1 -
CHEMBL399121 HESPERETIN 0.5 1 -
CHEMBL274318 FLAVANONE 1 -
CHEMBL73933 4'-HYDROXY-FLAVANONE 1 -
CHEMBL75897 2'-HYDROXY-FLAVANONE 1 -
CHEMBL28 APIGENIN 1 -
CHEMBL276915 7-HYDROXYFLAVONE 1 -
CHEMBL195033 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL150 KAEMPFEROL IC50 = 360.0 nM 6.44
CHEMBL50 QUERCETIN IC50 = 1540.0 nM 5.81
CHEMBL131921 BIOCHANIN IC50 = 9900.0 nM 5.00
CHEMBL9352 NARINGENIN IC50 = 14400.0 nM 4.84
CHEMBL44 GENISTEIN IC50 = 16500.0 nM 4.78
CHEMBL194399 ENDOSULFAN IC50 - -
CHEMBL51483 GOSSYPOL IC50 - -
CHEMBL230006 ENOXOLONE IC50 - -
CHEMBL8145 DAIDZEIN IC50 - -
CHEMBL117 CHRYSIN IC50 - -
CHEMBL275638 FLAVONE IC50 - -
CHEMBL399121 HESPERETIN IC50 - -
CHEMBL274318 FLAVANONE IC50 - -
CHEMBL73933 4'-HYDROXY-FLAVANONE IC50 - -
CHEMBL75897 2'-HYDROXY-FLAVANONE IC50 - -
CHEMBL28 APIGENIN IC50 > 20000.0 nM -
CHEMBL276915 7-HYDROXYFLAVONE IC50 > 20000.0 nM -
CHEMBL195033 IC50 - -