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Assay Detail

CHEMBL963642

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antagonist activity at recombinant GnRH receptor in CO2 asphyxiated rat primary pituitary cells assessed as reduction in (D-Trp6)LHRH-induced LH release
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Rattus norvegicus
Cell Type Pituitary gland cell
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Gonadotropin-releasing hormone receptor (CHEMBL3066)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

2-phenyl-4-piperazinylbenzimidazoles: orally active inhibitors of the gonadotropin releasing hormone (GnRH) receptor.
Pelletier JC, Chengalvala M, Cottom J, Feingold I, Garrick L, Green D, Hauze D, Huselton C, Jetter J, Kao W, Kopf GS, Lundquist JT, Mann C, Mehlmann J, Rogers J, Shanno L, Wrobel J.
Bioorg Med Chem (2008) 16 : 6617 -6640
PubMed 18511284 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 8 6.15 7.60

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL459215 1 7.60
CHEMBL516479 1 7.39
CHEMBL460480 1 7.23
CHEMBL459659 1 6.62
CHEMBL517881 1 6.32
CHEMBL460892 1 5.82
CHEMBL512893 1 4.12
CHEMBL472117 1 4.08

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL459215 IC50 = 25.0 nM 7.60
CHEMBL516479 IC50 = 41.0 nM 7.39
CHEMBL460480 IC50 = 59.0 nM 7.23
CHEMBL459659 IC50 = 240.0 nM 6.62
CHEMBL517881 IC50 = 480.0 nM 6.32
CHEMBL460892 IC50 = 1500.0 nM 5.82
CHEMBL512893 IC50 = 76000.0 nM 4.12
CHEMBL472117 IC50 = 83000.0 nM 4.08