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Assay Detail

CHEMBL964272

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human recombinant iNOS using 1 mM NADH and 2 uM L-arginine after 30 mins
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Nitric oxide synthase, inducible (CHEMBL4481)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design and synthesis of 2-amino-4-methylpyridine analogues as inhibitors for inducible nitric oxide synthase and in vivo evaluation of [18F]6-(2-fluoropropyl)-4-methyl-pyridin-2-amine as a potential PET tracer for inducible nitric oxide synthase.
Zhou D, Lee H, Rothfuss JM, Chen DL, Ponde DE, Welch MJ, Mach RH.
J Med Chem (2009) 52 : 2443 -2453
PubMed 19323559 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 12 6.50 7.24

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL443862 1 7.24
CHEMBL509542 1 6.77
CHEMBL294609 1 6.71
CHEMBL450726 1 6.66
CHEMBL501620 1 6.55
CHEMBL464556 1 6.16
CHEMBL447472 1 6.14
CHEMBL500364 1 5.75
CHEMBL478130 1 -
CHEMBL463939 1 -
CHEMBL477457 1 -
CHEMBL477458 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL443862 IC50 = 57.6 nM 7.24
CHEMBL509542 IC50 = 170.0 nM 6.77
CHEMBL294609 IC50 = 193.0 nM 6.71
CHEMBL450726 IC50 = 220.0 nM 6.66
CHEMBL501620 IC50 = 282.0 nM 6.55
CHEMBL464556 IC50 = 685.0 nM 6.16
CHEMBL447472 IC50 = 731.0 nM 6.14
CHEMBL500364 IC50 = 1776.0 nM 5.75
CHEMBL478130 IC50 > 5000.0 nM -
CHEMBL463939 IC50 > 5000.0 nM -
CHEMBL477457 IC50 > 5000.0 nM -
CHEMBL477458 IC50 > 5000.0 nM -