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Assay Detail

CHEMBL969583

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Inhibition of SHH in mouse Shh Light2 cells by GLI-responsive firefly luciferase reporter gene assay
11
Total Activities
11
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Cell Type Shh Light II
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Sonic hedgehog protein (CHEMBL5387)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Hedgehog-Gli signaling pathway inhibitors as anticancer agents.
Mahindroo N, Punchihewa C, Fujii N.
J Med Chem (2009) 52 : 3829 -3845
PubMed 19309080 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 9 6.83 8.52
EC50 2 6.85 7.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL473417 VISMODEGIB 4.0 1 8.52
CHEMBL514622 1 7.52
CHEMBL516246 1 7.40
CHEMBL470513 1 7.00
CHEMBL511979 1 7.00
CHEMBL470514 1 7.00
CHEMBL511978 1 7.00
CHEMBL254129 CYCLOPAMINE 1 6.30
CHEMBL471728 1 5.30
CHEMBL472994 1 5.30
CHEMBL472997 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL473417 VISMODEGIB IC50 = 3.0 nM 8.52
CHEMBL514622 IC50 = 30.0 nM 7.52
CHEMBL516246 EC50 = 40.0 nM 7.40
CHEMBL470513 IC50 = 100.0 nM 7.00
CHEMBL511979 IC50 = 100.0 nM 7.00
CHEMBL470514 IC50 = 100.0 nM 7.00
CHEMBL511978 IC50 = 100.0 nM 7.00
CHEMBL254129 CYCLOPAMINE EC50 = 500.0 nM 6.30
CHEMBL471728 IC50 = 5000.0 nM 5.30
CHEMBL472994 IC50 = 5000.0 nM 5.30
CHEMBL472997 IC50 < 1.0 nM -