Assay Detail
Functional
CHEMBL971541
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Agonist activity at N-terminal HA epitope-tagged wild type 3 human P2Y2 receptor expressed in human 1321N1 cells assessed as increase in intracellular calcium level
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Functional |
| Organism | Homo sapiens |
| Cell Type | 1321-N1 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Key determinants of nucleotide-activated G protein-coupled P2Y(2) receptor function revealed by chemical and pharmacological experiments, mutagenesis and homology modeling.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 5 | 7.00 | 7.09 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL336296 | URIDINE TRIPHOSPHATE | 3.0 | 1 | 7.09 |
| CHEMBL401735 | — | — | 1 | 7.09 |
| CHEMBL14249 | ADENOSINE TRIPHOSPHATE | 2.0 | 1 | 7.02 |
| CHEMBL221326 | DIQUAFOSOL | 4.0 | 1 | 6.95 |
| CHEMBL339385 | DIADENOSINE TETRAPHOSPHATE | — | 1 | 6.84 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL336296 | URIDINE TRIPHOSPHATE | EC50 | = | 80.4 | nM | 7.09 |
| CHEMBL401735 | — | EC50 | = | 80.4 | nM | 7.09 |
| CHEMBL14249 | ADENOSINE TRIPHOSPHATE | EC50 | = | 95.8 | nM | 7.02 |
| CHEMBL221326 | DIQUAFOSOL | EC50 | = | 112.0 | nM | 6.95 |
| CHEMBL339385 | DIADENOSINE TETRAPHOSPHATE | EC50 | = | 145.0 | nM | 6.84 |