Assay Detail
Binding
CHEMBL979821
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Binding affinity to Mycobacterium tuberculosis His4-tagged recombinant CYP51 F78L mutant expressed in Escherichia coli HMS174(DE3) by UV-visible spectral titration method
4
Total Activities
4
Compounds Tested
2
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Mycobacterium tuberculosis |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Sterol 14alpha-demethylase (CHEMBL5090) ↗| Type | SINGLE PROTEIN |
| Organism | Mycobacterium tuberculosis |
Publication
Small-molecule scaffolds for CYP51 inhibitors identified by high-throughput screening and defined by X-ray crystallography.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Kd | 3 | 4.50 | 4.71 |
| Activity | 1 | - | - |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL507264 | — | — | 1 | 4.71 |
| CHEMBL489925 | — | — | 1 | 4.63 |
| CHEMBL106 | FLUCONAZOLE | 4.0 | 1 | 4.16 |
| CHEMBL194859 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL507264 | — | Kd | = | 19500.0 | nM | 4.71 |
| CHEMBL489925 | — | Kd | = | 23200.0 | nM | 4.63 |
| CHEMBL106 | FLUCONAZOLE | Kd | = | 70000.0 | nM | 4.16 |
| CHEMBL194859 | — | Activity | - | — | - |