Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL982702

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human recombinant DAGL-alpha-mediated sn-1-[14C]oleoyl-2-arachidonoyl-glycerol hydrolysis to 2-AG overexpressed in african green monkey COS7 cell membrane by scintillation counting
22
Total Activities
22
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type COS-7
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Diacylglycerol lipase-alpha (CHEMBL5545)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Tetrahydrolipstatin analogues as modulators of endocannabinoid 2-arachidonoylglycerol metabolism.
Ortar G, Bisogno T, Ligresti A, Morera E, Nalli M, Di Marzo V.
J Med Chem (2008) 51 : 6970 -6979
PubMed 18831576 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 22 6.08 7.80

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL521157 1 7.80
CHEMBL457232 1 7.46
CHEMBL516323 1 7.37
CHEMBL484393 1 7.00
CHEMBL484215 1 6.37
CHEMBL521467 1 6.34
CHEMBL514886 1 6.22
CHEMBL458828 1 6.10
CHEMBL175247 ORLISTAT 4.0 1 6.00
CHEMBL484392 1 5.96
CHEMBL458827 1 5.55
CHEMBL458622 1 5.30
CHEMBL458972 1 4.60
CHEMBL484371 1 4.60
CHEMBL520494 1 4.60
CHEMBL453289 1 -
CHEMBL457736 1 -
CHEMBL451312 1 -
CHEMBL458108 1 -
CHEMBL458090 1 -
CHEMBL443980 1 -
CHEMBL458623 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL521157 IC50 = 16.0 nM 7.80
CHEMBL457232 IC50 = 35.0 nM 7.46
CHEMBL516323 IC50 = 43.0 nM 7.37
CHEMBL484393 IC50 = 100.0 nM 7.00
CHEMBL484215 IC50 = 430.0 nM 6.37
CHEMBL521467 IC50 = 460.0 nM 6.34
CHEMBL514886 IC50 = 600.0 nM 6.22
CHEMBL458828 IC50 = 800.0 nM 6.10
CHEMBL175247 ORLISTAT IC50 = 1000.0 nM 6.00
CHEMBL484392 IC50 = 1100.0 nM 5.96
CHEMBL458827 IC50 = 2800.0 nM 5.55
CHEMBL458622 IC50 = 5000.0 nM 5.30
CHEMBL458972 IC50 = 25000.0 nM 4.60
CHEMBL484371 IC50 = 25000.0 nM 4.60
CHEMBL520494 IC50 = 25000.0 nM 4.60
CHEMBL453289 IC50 > 25000.0 nM -
CHEMBL457736 IC50 > 25000.0 nM -
CHEMBL451312 IC50 > 25000.0 nM -
CHEMBL458108 IC50 > 25000.0 nM -
CHEMBL458090 IC50 > 25000.0 nM -
CHEMBL443980 IC50 > 25000.0 nM -
CHEMBL458623 IC50 > 25000.0 nM -