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Assay Detail

CHEMBL983764

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Escherichia coli dihydrofolate reductase
21
Total Activities
21
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Escherichia coli
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Dihydrofolate reductase (CHEMBL1809)
Type SINGLE PROTEIN
Organism Escherichia coli

Publication

The effect of 5-alkyl modification on the biological activity of pyrrolo[2,3-d]pyrimidine containing classical and nonclassical antifolates as inhibitors of dihydrofolate reductase and as antitumor and/or antiopportunistic infection agents.
Gangjee A, Jain HD, Queener SF, Kisliuk RL.
J Med Chem (2008) 51 : 4589 -4600
PubMed 18605720 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 21 6.43 8.18

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL34259 METHOTREXATE 4.0 1 8.18
CHEMBL22 TRIMETHOPRIM 4.0 1 7.70
CHEMBL516128 1 6.96
CHEMBL477566 1 6.96
CHEMBL478403 1 6.89
CHEMBL477151 1 6.85
CHEMBL478404 1 6.82
CHEMBL476945 1 6.82
CHEMBL477150 1 6.54
CHEMBL476745 1 6.30
CHEMBL478213 1 6.22
CHEMBL477790 1 6.21
CHEMBL514219 1 5.89
CHEMBL516891 1 5.89
CHEMBL476744 1 5.85
CHEMBL515312 1 5.82
CHEMBL477789 1 5.82
CHEMBL36 PYRIMETHAMINE 4.0 1 5.70
CHEMBL478191 1 5.68
CHEMBL478192 1 5.52
CHEMBL225072 PEMETREXED 4.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL34259 METHOTREXATE IC50 = 6.6 nM 8.18
CHEMBL22 TRIMETHOPRIM IC50 = 20.0 nM 7.70
CHEMBL516128 IC50 = 110.0 nM 6.96
CHEMBL477566 IC50 = 110.0 nM 6.96
CHEMBL478403 IC50 = 130.0 nM 6.89
CHEMBL477151 IC50 = 140.0 nM 6.85
CHEMBL478404 IC50 = 150.0 nM 6.82
CHEMBL476945 IC50 = 150.0 nM 6.82
CHEMBL477150 IC50 = 290.0 nM 6.54
CHEMBL476745 IC50 = 500.0 nM 6.30
CHEMBL478213 IC50 = 600.0 nM 6.22
CHEMBL477790 IC50 = 610.0 nM 6.21
CHEMBL514219 IC50 = 1300.0 nM 5.89
CHEMBL516891 IC50 = 1300.0 nM 5.89
CHEMBL476744 IC50 = 1400.0 nM 5.85
CHEMBL515312 IC50 = 1500.0 nM 5.82
CHEMBL477789 IC50 = 1500.0 nM 5.82
CHEMBL36 PYRIMETHAMINE IC50 = 2000.0 nM 5.70
CHEMBL478191 IC50 = 2100.0 nM 5.68
CHEMBL478192 IC50 = 3000.0 nM 5.52
CHEMBL225072 PEMETREXED IC50 = 230000.0 nM -