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Assay Detail

CHEMBL985687

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of mouse COX2 in LPS-stimulated mouse J774 cells assessed as PGE2 production by radioimmunoassay
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Mus musculus
Cell Type J774
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Prostaglandin G/H synthase 2 (CHEMBL4321)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Synthesis, biological evaluation, and enzyme docking simulations of 1,5-diarylpyrrole-3-alkoxyethyl ethers as selective cyclooxygenase-2 inhibitors endowed with anti-inflammatory and antinociceptive activity.
Anzini M, Rovini M, Cappelli A, Vomero S, Manetti F, Botta M, Sautebin L, Rossi A, Pergola C, Ghelardini C, Norcini M, Giordani A, Makovec F, Anzellotti P, Patrignani P, Biava M.
J Med Chem (2008) 51 : 4476 -4481
PubMed 18598017 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 12 7.31 7.82

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL477362 1 7.82
CHEMBL517675 1 7.75
CHEMBL451358 1 7.75
CHEMBL479054 1 7.52
CHEMBL191941 1 7.40
CHEMBL476536 1 7.33
CHEMBL516289 1 7.32
CHEMBL478415 1 7.31
CHEMBL192789 1 7.22
CHEMBL515478 1 7.07
CHEMBL479053 1 6.96
CHEMBL363398 1 6.32

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL477362 IC50 = 15.0 nM 7.82
CHEMBL517675 IC50 = 18.0 nM 7.75
CHEMBL451358 IC50 = 18.0 nM 7.75
CHEMBL479054 IC50 = 30.0 nM 7.52
CHEMBL191941 IC50 = 40.0 nM 7.40
CHEMBL476536 IC50 = 47.0 nM 7.33
CHEMBL516289 IC50 = 48.0 nM 7.32
CHEMBL478415 IC50 = 49.0 nM 7.31
CHEMBL192789 IC50 = 60.0 nM 7.22
CHEMBL515478 IC50 = 85.0 nM 7.07
CHEMBL479053 IC50 = 110.0 nM 6.96
CHEMBL363398 IC50 = 480.0 nM 6.32