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Assay Detail

CHEMBL986308

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Histamine H4 receptor (CHEMBL4468)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Rotationally constrained 2,4-diamino-5,6-disubstituted pyrimidines: a new class of histamine H4 receptor antagonists with improved druglikeness and in vivo efficacy in pain and inflammation models.
Cowart MD, Altenbach RJ, Liu H, Hsieh GC, Drizin I, Milicic I, Miller TR, Witte DG, Wishart N, Fix-Stenzel SR, McPherson MJ, Adair RM, Wetter JM, Bettencourt BM, Marsh KC, Sullivan JP, Honore P, Esbenshade TA, Brioni JD.
J Med Chem (2008) 51 : 6547 -6557
PubMed 18817367 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 14 8.07 8.84

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL523225 1 8.84
CHEMBL129198 JNJ-7777120 1 8.57
CHEMBL522673 1 8.44
CHEMBL502347 1 8.42
CHEMBL503605 1 8.40
CHEMBL492231 1 8.35
CHEMBL521995 1 8.32
CHEMBL521996 1 8.29
CHEMBL494093 1 8.27
CHEMBL509540 1 8.12
CHEMBL493281 1 8.04
CHEMBL492884 1 7.85
CHEMBL494678 1 6.56
CHEMBL494890 1 6.47

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL523225 Ki = 1.445 nM 8.84
CHEMBL129198 JNJ-7777120 Ki = 2.692 nM 8.57
CHEMBL522673 Ki = 3.631 nM 8.44
CHEMBL502347 Ki = 3.802 nM 8.42
CHEMBL503605 Ki = 3.981 nM 8.40
CHEMBL492231 Ki = 4.467 nM 8.35
CHEMBL521995 Ki = 4.786 nM 8.32
CHEMBL521996 Ki = 5.129 nM 8.29
CHEMBL494093 Ki = 5.37 nM 8.27
CHEMBL509540 Ki = 7.586 nM 8.12
CHEMBL493281 Ki = 9.12 nM 8.04
CHEMBL492884 Ki = 14.13 nM 7.85
CHEMBL494678 Ki = 275.42 nM 6.56
CHEMBL494890 Ki = 338.84 nM 6.47