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Assay Detail

CHEMBL990676

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human recombinant caspase 3 assessed as accumulation of 7-amino-4-methylcoumarin substrate
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Caspase-3 (CHEMBL2334)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design, synthesis, and biological characterization of a caspase 3/7 selective isatin labeled with 2-[18F]fluoroethylazide.
Smith G, Glaser M, Perumal M, Nguyen QD, Shan B, Arstad E, Aboagye EO.
J Med Chem (2008) 51 : 8057 -8067
PubMed 19049429 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 14 7.79 9.30

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL456799 1 9.30
CHEMBL456593 1 8.64
CHEMBL515506 1 8.26
CHEMBL516941 1 7.91
CHEMBL518382 1 7.90
CHEMBL456592 1 7.78
CHEMBL456798 1 7.78
CHEMBL461249 1 7.77
CHEMBL384023 1 7.60
CHEMBL516490 1 7.58
CHEMBL439753 1 7.38
CHEMBL515661 1 7.30
CHEMBL372607 1 7.11
CHEMBL461248 1 6.70

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL456799 EC50 = 0.5 nM 9.30
CHEMBL456593 EC50 = 2.3 nM 8.64
CHEMBL515506 EC50 = 5.5 nM 8.26
CHEMBL516941 EC50 = 12.4 nM 7.91
CHEMBL518382 EC50 = 12.6 nM 7.90
CHEMBL456592 EC50 = 16.8 nM 7.78
CHEMBL456798 EC50 = 16.7 nM 7.78
CHEMBL461249 EC50 = 17.0 nM 7.77
CHEMBL384023 EC50 = 25.3 nM 7.60
CHEMBL516490 EC50 = 26.1 nM 7.58
CHEMBL439753 EC50 = 41.8 nM 7.38
CHEMBL515661 EC50 = 50.1 nM 7.30
CHEMBL372607 EC50 = 78.6 nM 7.11
CHEMBL461248 EC50 = 199.5 nM 6.70