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Assay Detail

CHEMBL994494

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [125I]Tyro-rat/human CRF from CRF1 receptor in rat cortical membrane by gamma counting
30
Total Activities
30
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Corticotropin-releasing factor receptor 1 (CHEMBL4649)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Synthesis and structure-activity relationships of 8-(pyrid-3-yl)pyrazolo[1,5-a]-1,3,5-triazines: potent, orally bioavailable corticotropin releasing factor receptor-1 (CRF1) antagonists.
Gilligan PJ, Clarke T, He L, Lelas S, Li YW, Heman K, Fitzgerald L, Miller K, Zhang G, Marshall A, Krause C, McElroy JF, Ward K, Zeller K, Wong H, Bai S, Saye J, Grossman S, Zaczek R, Arneric SP, Hartig P, Robertson D, Trainor G.
J Med Chem (2009) 52 : 3084 -3092
PubMed 19361209 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 30 7.96 8.80

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL519159 1 8.80
CHEMBL483162 1 8.62
CHEMBL482752 1 8.52
CHEMBL516242 1 8.51
CHEMBL519967 1 8.51
CHEMBL473069 1 8.46
CHEMBL474289 1 8.35
CHEMBL500013 1 8.31
CHEMBL473070 1 8.29
CHEMBL473277 1 8.24
CHEMBL482950 PEXACERFONT 3.0 1 8.21
CHEMBL475507 1 8.18
CHEMBL474288 1 8.15
CHEMBL483350 1 8.09
CHEMBL514465 1 8.07
CHEMBL473276 1 7.92
CHEMBL482543 1 7.89
CHEMBL520955 1 7.86
CHEMBL482751 1 7.84
CHEMBL473275 1 7.83
CHEMBL483349 1 7.75
CHEMBL484930 1 7.62
CHEMBL520344 1 7.61
CHEMBL474491 1 7.59
CHEMBL482542 1 7.48
CHEMBL474493 1 7.24
CHEMBL453001 1 7.09
CHEMBL482544 1 7.04
CHEMBL474492 1 6.88
CHEMBL515583 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL519159 IC50 = 1.6 nM 8.80
CHEMBL483162 IC50 = 2.4 nM 8.62
CHEMBL482752 IC50 = 3.0 nM 8.52
CHEMBL516242 IC50 = 3.1 nM 8.51
CHEMBL519967 IC50 = 3.1 nM 8.51
CHEMBL473069 IC50 = 3.5 nM 8.46
CHEMBL474289 IC50 = 4.5 nM 8.35
CHEMBL500013 IC50 = 4.9 nM 8.31
CHEMBL473070 IC50 = 5.1 nM 8.29
CHEMBL473277 IC50 = 5.7 nM 8.24
CHEMBL482950 PEXACERFONT IC50 = 6.1 nM 8.21
CHEMBL475507 IC50 = 6.6 nM 8.18
CHEMBL474288 IC50 = 7.0 nM 8.15
CHEMBL483350 IC50 = 8.1 nM 8.09
CHEMBL514465 IC50 = 8.5 nM 8.07
CHEMBL473276 IC50 = 12.0 nM 7.92
CHEMBL482543 IC50 = 12.8 nM 7.89
CHEMBL520955 IC50 = 13.8 nM 7.86
CHEMBL482751 IC50 = 14.5 nM 7.84
CHEMBL473275 IC50 = 14.9 nM 7.83
CHEMBL483349 IC50 = 17.6 nM 7.75
CHEMBL484930 IC50 = 23.8 nM 7.62
CHEMBL520344 IC50 = 24.8 nM 7.61
CHEMBL474491 IC50 = 25.6 nM 7.59
CHEMBL482542 IC50 = 32.8 nM 7.48
CHEMBL474493 IC50 = 58.0 nM 7.24
CHEMBL453001 IC50 = 81.6 nM 7.09
CHEMBL482544 IC50 = 91.9 nM 7.04
CHEMBL474492 IC50 = 133.3 nM 6.88
CHEMBL515583 IC50 > 10000.0 nM -