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Assay Detail

CHEMBL995892

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Kca2.3 channel expressed in HEK293 cells by thallium flux assay
25
Total Activities
25
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Cell Type HEK293
Confidence 8 — Homologous single protein target
Curated By Intermediate

Publication

Preliminary SAR studies on non-apamin-displacing 4-(aminomethylaryl)pyrrazolopyrimidine K(Ca) channel blockers.
Gentles RG, Hu S, Huang Y, Grant-Young K, Poss MA, Andres C, Fiedler T, Knox R, Lodge N, Weaver CD, Harden DG.
Bioorg Med Chem Lett (2008) 18 : 5694 -5697
PubMed 18824351 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 23 5.51 9.78
Activity 2 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL525408 APAMIN 1 9.78
CHEMBL472452 1 6.46
CHEMBL469732 1 6.00
CHEMBL452406 1 5.96
CHEMBL469756 1 5.28
CHEMBL513544 1 5.12
CHEMBL471826 1 5.10
CHEMBL509297 1 5.00
CHEMBL511783 1 4.97
CHEMBL469540 1 4.96
CHEMBL470804 1 4.84
CHEMBL469539 1 4.84
CHEMBL471192 1 4.81
CHEMBL443165 1 4.80
CHEMBL513561 1 4.72
CHEMBL471852 1 -
CHEMBL472028 1 -
CHEMBL512698 1 -
CHEMBL471193 1 -
CHEMBL510479 1 -
CHEMBL471194 1 -
CHEMBL469731 1 -
CHEMBL445782 1 -
CHEMBL472402 1 -
CHEMBL502413 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL525408 APAMIN IC50 = 0.168 nM 9.78
CHEMBL472452 IC50 = 350.0 nM 6.46
CHEMBL469732 IC50 = 1000.0 nM 6.00
CHEMBL452406 IC50 = 1100.0 nM 5.96
CHEMBL469756 IC50 = 5200.0 nM 5.28
CHEMBL513544 IC50 = 7500.0 nM 5.12
CHEMBL471826 IC50 = 7900.0 nM 5.10
CHEMBL509297 IC50 = 10000.0 nM 5.00
CHEMBL511783 IC50 = 10700.0 nM 4.97
CHEMBL469540 IC50 = 11000.0 nM 4.96
CHEMBL470804 IC50 = 14600.0 nM 4.84
CHEMBL469539 IC50 = 14600.0 nM 4.84
CHEMBL471192 IC50 = 15400.0 nM 4.81
CHEMBL443165 IC50 = 15900.0 nM 4.80
CHEMBL513561 IC50 = 19100.0 nM 4.72
CHEMBL471852 IC50 > 30000.0 nM -
CHEMBL472028 IC50 > 30000.0 nM -
CHEMBL512698 IC50 > 30000.0 nM -
CHEMBL471193 IC50 > 30000.0 nM -
CHEMBL510479 IC50 > 30000.0 nM -
CHEMBL471194 IC50 > 30000.0 nM -
CHEMBL469731 Activity - -
CHEMBL445782 IC50 > 30000.0 nM -
CHEMBL472402 Activity - -
CHEMBL502413 IC50 > 30000.0 nM -