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Assay Detail

CHEMBL996091

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Functional
Agonist activity at human PAR2 expressed in HEK293T cells assessed as effect on intracellular calcium mobilization by R-SAT assay
14
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HEK-293T
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Proteinase-activated receptor 2 (CHEMBL5963)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of potent and selective small-molecule PAR-2 agonists.
Seitzberg JG, Knapp AE, Lund BW, Mandrup Bertozzi S, Currier EA, Ma JN, Sherbukhin V, Burstein ES, Olsson R.
J Med Chem (2008) 51 : 5490 -5493
PubMed 18720984 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 14 6.32 7.60

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL459723 1 7.60
CHEMBL509819 1 7.60
CHEMBL494502 3 7.50
CHEMBL461639 1 7.30
CHEMBL459509 1 6.90
CHEMBL493076 1 6.70
CHEMBL493704 1 5.60
CHEMBL459724 1 5.20
CHEMBL494303 1 5.20
CHEMBL494503 1 5.10
CHEMBL238078 1 5.00
CHEMBL493494 1 5.00

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL459723 EC50 = 25.12 nM 7.60
CHEMBL509819 EC50 = 25.12 nM 7.60
CHEMBL494502 EC50 = 31.62 nM 7.50
CHEMBL494502 EC50 = 31.62 nM 7.50
CHEMBL461639 EC50 = 50.12 nM 7.30
CHEMBL459509 EC50 = 125.89 nM 6.90
CHEMBL493076 EC50 = 199.53 nM 6.70
CHEMBL493704 EC50 = 2511.89 nM 5.60
CHEMBL459724 EC50 = 6309.57 nM 5.20
CHEMBL494303 EC50 = 6309.57 nM 5.20
CHEMBL494503 EC50 = 7943.28 nM 5.10
CHEMBL238078 EC50 = 10000.0 nM 5.00
CHEMBL493494 EC50 = 10000.0 nM 5.00
CHEMBL494502 EC50 > 10000.0 nM -