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Assay Detail

CHEMBL996771

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Inhibition of glucagon-stimulated glucose production in human primary hepatocytes preincubated for 10 mins before glucagon addition by NADH-linked hexokinase assay
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type Hepatocyte
Confidence 1 — Organism
Curated By Autocuration

Target

Hepatocyte (CHEMBL613690)
Type CELL-LINE
Organism Homo sapiens

Publication

Human glucagon receptor antagonists with thiazole cores. A novel series with superior pharmacokinetic properties.
Madsen P, Kodra JT, Behrens C, Nishimura E, Jeppesen CB, Pridal L, Andersen B, Knudsen LB, Valcarce-Aspegren C, Guldbrandt M, Christensen IT, Jørgensen AS, Ynddal L, Brand CL, Bagger MA, Lau J.
J Med Chem (2009) 52 : 2989 -3000
PubMed 19385613 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 8 5.48 6.16

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL455323 1 6.16
CHEMBL471580 1 5.57
CHEMBL519776 1 5.55
CHEMBL501628 1 5.44
CHEMBL492813 1 5.40
CHEMBL470729 1 5.17
CHEMBL446821 1 5.07
CHEMBL492605 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL455323 IC50 = 700.0 nM 6.16
CHEMBL471580 IC50 = 2700.0 nM 5.57
CHEMBL519776 IC50 = 2800.0 nM 5.55
CHEMBL501628 IC50 = 3600.0 nM 5.44
CHEMBL492813 IC50 = 4000.0 nM 5.40
CHEMBL470729 IC50 = 6700.0 nM 5.17
CHEMBL446821 IC50 = 8600.0 nM 5.07
CHEMBL492605 IC50 < 20000.0 nM -