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Assay Detail

CHEMBL997159

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of wild type chicken N-terminal His-tagged cSrc expressed in Escherichia coli BL21 (DE3)
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Gallus gallus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Proto-oncogene tyrosine-protein kinase Src (CHEMBL3655)
Type SINGLE PROTEIN
Organism Gallus gallus

Publication

Hybrid compound design to overcome the gatekeeper T338M mutation in cSrc.
Getlik M, Grütter C, Simard JR, Klüter S, Rabiller M, Rode HB, Robubi A, Rauh D.
J Med Chem (2009) 52 : 3915 -3926
PubMed 19462975 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 9 6.58 9.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5416410 DASATINIB 4.0 1 9.40
CHEMBL510516 1 7.85
CHEMBL497033 1 7.68
CHEMBL526930 1 7.15
CHEMBL526560 1 6.68
CHEMBL497615 1 6.63
CHEMBL188762 1 5.19
CHEMBL498947 1 4.49
CHEMBL522387 1 4.19

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5416410 DASATINIB IC50 = 0.4 nM 9.40
CHEMBL510516 IC50 = 14.0 nM 7.85
CHEMBL497033 IC50 = 21.0 nM 7.68
CHEMBL526930 IC50 = 71.0 nM 7.15
CHEMBL526560 IC50 = 207.0 nM 6.68
CHEMBL497615 IC50 = 235.0 nM 6.63
CHEMBL188762 IC50 = 6400.0 nM 5.19
CHEMBL498947 IC50 = 32100.0 nM 4.49
CHEMBL522387 IC50 = 64100.0 nM 4.19