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Assay Detail

CHEMBL997164

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Activity of human N-terminal His-tagged p38alpha expressed in Escherichia coli BL21 (DE3)
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Mitogen-activated protein kinase 14 (CHEMBL260)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Hybrid compound design to overcome the gatekeeper T338M mutation in cSrc.
Getlik M, Grütter C, Simard JR, Klüter S, Rabiller M, Rode HB, Robubi A, Rauh D.
J Med Chem (2009) 52 : 3915 -3926
PubMed 19462975 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 9 6.99 7.92

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL522387 1 7.92
CHEMBL526560 1 7.30
CHEMBL498947 1 7.26
CHEMBL497615 1 7.13
CHEMBL526930 1 6.90
CHEMBL510516 1 6.61
CHEMBL497033 1 6.47
CHEMBL5416410 DASATINIB 4.0 1 6.30
CHEMBL188762 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL522387 Kd = 12.0 nM 7.92
CHEMBL526560 Kd = 50.0 nM 7.30
CHEMBL498947 Kd = 55.0 nM 7.26
CHEMBL497615 Kd = 74.0 nM 7.13
CHEMBL526930 Kd = 127.0 nM 6.90
CHEMBL510516 Kd = 245.0 nM 6.61
CHEMBL497033 Kd = 342.0 nM 6.47
CHEMBL5416410 DASATINIB Kd = 495.0 nM 6.30
CHEMBL188762 Kd - -