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Assay Detail

CHEMBL997224

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [125I][Nle4,Dphe7]-alpha-MSH from human MC4R expressed in HEK293 cells
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Melanocortin receptor 4 (CHEMBL259)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Substitution of arginine with proline and proline derivatives in melanocyte-stimulating hormones leads to selectivity for human melanocortin 4 receptor.
Qu H, Cai M, Mayorov AV, Grieco P, Zingsheim M, Trivedi D, Hruby VJ.
J Med Chem (2009) 52 : 3627 -3635
PubMed 19473029 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 14 8.18 9.22

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2096742 1 9.22
CHEMBL500743 1 9.22
CHEMBL430239 MELATONAN 1 8.96
CHEMBL503229 1 8.92
CHEMBL501592 1 8.82
CHEMBL502300 1 8.60
CHEMBL524861 1 8.24
CHEMBL509582 1 8.14
CHEMBL501642 1 7.96
CHEMBL508501 1 7.64
CHEMBL194552 1 7.48
CHEMBL444493 1 7.41
CHEMBL455070 1 7.30
CHEMBL526334 1 6.57

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2096742 IC50 = 0.6 nM 9.22
CHEMBL500743 IC50 = 0.6 nM 9.22
CHEMBL430239 MELATONAN IC50 = 1.1 nM 8.96
CHEMBL503229 IC50 = 1.2 nM 8.92
CHEMBL501592 IC50 = 1.5 nM 8.82
CHEMBL502300 IC50 = 2.5 nM 8.60
CHEMBL524861 IC50 = 5.7 nM 8.24
CHEMBL509582 IC50 = 7.2 nM 8.14
CHEMBL501642 IC50 = 11.0 nM 7.96
CHEMBL508501 IC50 = 23.0 nM 7.64
CHEMBL194552 IC50 = 33.0 nM 7.48
CHEMBL444493 IC50 = 39.0 nM 7.41
CHEMBL455070 IC50 = 50.0 nM 7.30
CHEMBL526334 IC50 = 270.0 nM 6.57