Assay Detail
Binding
CHEMBL999925
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of hypoxia-induced HIF1 activation in human HeLa cells by luciferase reporter gene assay
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | HeLa |
| Confidence | 5 — Multiple protein complex / RNA |
| Curated By | Autocuration |
Target
Hypoxia inducible factors; HIF-1-alpha, HIF-2-alpha (CHEMBL2221345) ↗| Type | PROTEIN FAMILY |
| Organism | Homo sapiens |
Publication
Terpenoid tetrahydroisoquinoline alkaloids emetine, klugine, and isocephaeline inhibit the activation of hypoxia-inducible factor-1 in breast tumor cells.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 2 | 7.26 | 7.44 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL123292 | CYCLOHEXIMIDE | 2.0 | 1 | 7.44 |
| CHEMBL50588 | EMETINE | 4.0 | 1 | 7.08 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL123292 | CYCLOHEXIMIDE | IC50 | = | 36.0 | nM | 7.44 |
| CHEMBL50588 | EMETINE | IC50 | = | 83.0 | nM | 7.08 |