Assay Detail
Functional
CHEMBL1006812
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Inhibition of recombinant human TNF-alpha-induced cytotoxicity of mouse L929 cells assessed as survivality preincubated for 15 mins before TNFalpha addition measured after 24 hrs by [methyl-3H]thymidine incorporation assay
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Functional |
| Organism | Mus musculus |
| Cell Type | L929 |
| Confidence | 1 — Organism |
| Curated By | Autocuration |
Publication
Flavonoids as inhibitors or enhancers of the cytotoxicity of tumor necrosis factor-alpha in L-929 tumor cells.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 12 | 4.61 | 5.22 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL8996 | ERIODICTYOL | — | 1 | 5.22 |
| CHEMBL52 | NORDIHYDROGUAIARETIC ACID | — | 1 | 4.72 |
| CHEMBL150 | KAEMPFEROL | 1.0 | 1 | 4.60 |
| CHEMBL50 | QUERCETIN | 3.0 | 1 | 4.58 |
| CHEMBL309490 | GALANGIN | — | 1 | 4.30 |
| CHEMBL164 | MYRICETIN | — | 1 | 4.23 |
| CHEMBL583912 | (-)-EPICATECHIN | 2.0 | 1 | - |
| CHEMBL40919 | KAEMPFERIDE | — | 1 | - |
| CHEMBL151 | LUTEOLIN | 2.0 | 1 | - |
| CHEMBL28626 | MORIN | — | 1 | - |
| CHEMBL226335 | RUTIN | 3.0 | 1 | - |
| CHEMBL66 | (+)-TAXIFOLIN | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL8996 | ERIODICTYOL | EC50 | = | 6000.0 | nM | 5.22 |
| CHEMBL52 | NORDIHYDROGUAIARETIC ACID | EC50 | = | 19000.0 | nM | 4.72 |
| CHEMBL150 | KAEMPFEROL | EC50 | = | 25000.0 | nM | 4.60 |
| CHEMBL50 | QUERCETIN | EC50 | = | 26000.0 | nM | 4.58 |
| CHEMBL309490 | GALANGIN | EC50 | = | 50000.0 | nM | 4.30 |
| CHEMBL164 | MYRICETIN | EC50 | = | 59000.0 | nM | 4.23 |
| CHEMBL583912 | (-)-EPICATECHIN | EC50 | = | 350000.0 | nM | - |
| CHEMBL40919 | KAEMPFERIDE | EC50 | = | 140000.0 | nM | - |
| CHEMBL151 | LUTEOLIN | EC50 | > | 250000.0 | nM | - |
| CHEMBL28626 | MORIN | EC50 | = | 115000.0 | nM | - |
| CHEMBL226335 | RUTIN | EC50 | > | 250000.0 | nM | - |
| CHEMBL66 | (+)-TAXIFOLIN | EC50 | = | 240000.0 | nM | - |