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Assay Detail

CHEMBL1011807

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]UR-MK114 from neuropeptide Y1 receptor in human SK-N-MC cells
9
Total Activities
5
Compounds Tested
3
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type SK-N-MC
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Neuropeptide Y receptor type 1 (CHEMBL4777)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Guanidine-acylguanidine bioisosteric approach in the design of radioligands: synthesis of a tritium-labeled N(G)-propionylargininamide ([3H]-UR-MK114) as a highly potent and selective neuropeptide Y Y1 receptor antagonist.
Keller M, Pop N, Hutzler C, Beck-Sickinger AG, Bernhardt G, Buschauer A.
J Med Chem (2008) 51 : 8168 -8172
PubMed 19053784 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 5 9.42 10.00
IC50 3 8.70 9.01
Kd 1 8.92 8.92

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL511460 1 10.00
CHEMBL508974 1 10.00
CHEMBL267633 NEUROPEPTIDE-Y 2 9.30
CHEMBL470495 3 9.00
CHEMBL332347 2 8.82

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL511460 Ki = 0.1 nM 10.00
CHEMBL508974 Ki = 0.1 nM 10.00
CHEMBL267633 NEUROPEPTIDE-Y Ki = 0.5 nM 9.30
CHEMBL267633 NEUROPEPTIDE-Y IC50 = 0.9772 nM 9.01
CHEMBL470495 Ki = 1.0 nM 9.00
CHEMBL470495 Kd = 1.2 nM 8.92
CHEMBL332347 Ki = 1.5 nM 8.82
CHEMBL470495 IC50 = 2.239 nM 8.65
CHEMBL332347 IC50 = 3.631 nM 8.44