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Assay Detail

CHEMBL1015373

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antagonist activity at human cloned muscarinic M3 receptor expressed in CHO cells assessed as inhibition of acetylcholine-induced calcium mobilization by single concentration kinetic based-FLIPR assay
22
Total Activities
22
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type CHO
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Muscarinic acetylcholine receptor M3 (CHEMBL245)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of novel 1-azoniabicyclo[2.2.2]octane muscarinic acetylcholine receptor antagonists.
Lainé DI, McCleland B, Thomas S, Neipp C, Underwood B, Dufour J, Widdowson KL, Palovich MR, Blaney FE, Foley JJ, Webb EF, Luttmann MA, Burman M, Belmonte K, Salmon M.
J Med Chem (2009) 52 : 2493 -2505
PubMed 19317446 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 22 9.56 10.60

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL489212 1 10.60
CHEMBL523299 UMECLIDINIUM BROMIDE 4.0 1 10.50
CHEMBL490237 1 10.40
CHEMBL523957 1 10.40
CHEMBL489211 1 10.40
CHEMBL522081 1 10.20
CHEMBL523474 1 10.20
CHEMBL521781 1 10.10
CHEMBL476434 1 10.00
CHEMBL524119 1 9.90
CHEMBL489625 1 9.60
CHEMBL489032 1 9.40
CHEMBL523598 1 9.40
CHEMBL490243 1 9.40
CHEMBL484475 1 9.30
CHEMBL490244 1 9.30
CHEMBL522418 1 9.10
CHEMBL519652 1 8.70
CHEMBL490236 1 8.50
CHEMBL490242 1 8.50
CHEMBL491246 1 8.40
CHEMBL490651 1 8.00

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL489212 Kd = 0.02512 nM 10.60
CHEMBL523299 UMECLIDINIUM BROMIDE Kd = 0.03162 nM 10.50
CHEMBL490237 Kd = 0.03981 nM 10.40
CHEMBL523957 Kd = 0.03981 nM 10.40
CHEMBL489211 Kd = 0.03981 nM 10.40
CHEMBL522081 Kd = 0.0631 nM 10.20
CHEMBL523474 Kd = 0.0631 nM 10.20
CHEMBL521781 Kd = 0.07943 nM 10.10
CHEMBL476434 Kd = 0.1 nM 10.00
CHEMBL524119 Kd = 0.1259 nM 9.90
CHEMBL489625 Kd = 0.2512 nM 9.60
CHEMBL489032 Kd = 0.3981 nM 9.40
CHEMBL523598 Kd = 0.3981 nM 9.40
CHEMBL490243 Kd = 0.3981 nM 9.40
CHEMBL484475 Kd = 0.5012 nM 9.30
CHEMBL490244 Kd = 0.5012 nM 9.30
CHEMBL522418 Kd = 0.7943 nM 9.10
CHEMBL519652 Kd = 1.995 nM 8.70
CHEMBL490236 Kd = 3.162 nM 8.50
CHEMBL490242 Kd = 3.162 nM 8.50
CHEMBL491246 Kd = 3.981 nM 8.40
CHEMBL490651 Kd = 10.0 nM 8.00