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Assay Detail

CHEMBL1022296

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human recombinant FAAH expressed in HEK293 cells preincubated for 30 mins before substrate addition by [3H]anandamide carbon filtration assay
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Fatty-acid amide hydrolase 1 (CHEMBL2243)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis and evaluation of benzothiazole-based analogues as novel, potent, and selective fatty acid amide hydrolase inhibitors.
Wang X, Sarris K, Kage K, Zhang D, Brown SP, Kolasa T, Surowy C, El Kouhen OF, Muchmore SW, Brioni JD, Stewart AO.
J Med Chem (2009) 52 : 170 -180
PubMed 19072118 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 3 7.69 7.80

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL184238 URB-597 1.0 1 7.80
CHEMBL367966 1 7.80
CHEMBL460340 1 7.47

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL184238 URB-597 IC50 = 16.0 nM 7.80
CHEMBL367966 IC50 = 16.0 nM 7.80
CHEMBL460340 IC50 = 34.0 nM 7.47