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Assay Detail

CHEMBL1059448

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Agonist activity at rat recombinant GluR4 flip isomer expressed in HEK293 cells assessed as increase in intracellular calcium level by Fluo-4/AM assay
6
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Rattus norvegicus
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Intermediate

Target

Glutamate receptor 4 (CHEMBL3505)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

The glutamate receptor GluR5 agonist (S)-2-amino-3-(3-hydroxy-7,8-dihydro-6H-cyclohepta[d]isoxazol-4-yl)propionic acid and the 8-methyl analogue: synthesis, molecular pharmacology, and biostructural characterization.
Clausen RP, Naur P, Kristensen AS, Greenwood JR, Strange M, Bräuner-Osborne H, Jensen AA, Nielsen AS, Geneser U, Ringgaard LM, Nielsen B, Pickering DS, Brehm L, Gajhede M, Krogsgaard-Larsen P, Kastrup JS.
J Med Chem (2009) 52 : 4911 -4922
PubMed 19588945 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 6 4.67 5.17

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL28472 1 5.17
CHEMBL575060 GLUTAMIC ACID 3.0 1 4.70
CHEMBL553028 2 4.46
CHEMBL275040 KAINIC ACID 2.0 1 4.33
CHEMBL27130 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL28472 EC50 = 6700.0 nM 5.17
CHEMBL575060 GLUTAMIC ACID EC50 = 20000.0 nM 4.70
CHEMBL553028 EC50 = 35000.0 nM 4.46
CHEMBL275040 KAINIC ACID EC50 = 47000.0 nM 4.33
CHEMBL27130 EC50 = 150.0 % -
CHEMBL553028 EC50 = 158489.32 nM -