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Assay Detail

CHEMBL1073247

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antagonist activity at mouse EP3alpha receptor expressed in CHo cells assessed as inhibition of PGE2-stimulated intracellular calcium concentration
19
Total Activities
19
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Cell Type CHO
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Prostaglandin E2 receptor EP3 subtype (CHEMBL4336)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Discovery of a series of acrylic acids and their derivatives as chemical leads for selective EP3 receptor antagonists.
Asada M, Obitsu T, Nagase T, Sugimoto I, Yamaura Y, Sato K, Narita M, Ohuchida S, Nakai H, Toda M.
Bioorg Med Chem (2009) 17 : 6567 -6582
PubMed 19700331 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 19 6.27 7.66

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL605833 1 7.66
CHEMBL596515 1 7.30
CHEMBL595632 1 7.14
CHEMBL593041 1 6.85
CHEMBL594365 1 6.82
CHEMBL594210 1 6.48
CHEMBL603653 1 6.40
CHEMBL595157 1 6.34
CHEMBL595830 1 6.27
CHEMBL604897 1 6.24
CHEMBL593526 1 6.22
CHEMBL595159 1 5.92
CHEMBL603625 1 5.82
CHEMBL603633 1 5.58
CHEMBL596499 1 5.52
CHEMBL594423 1 5.51
CHEMBL603227 1 5.46
CHEMBL593764 1 5.32
CHEMBL593260 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL605833 IC50 = 22.0 nM 7.66
CHEMBL596515 IC50 = 50.0 nM 7.30
CHEMBL595632 IC50 = 73.0 nM 7.14
CHEMBL593041 IC50 = 140.0 nM 6.85
CHEMBL594365 IC50 = 150.0 nM 6.82
CHEMBL594210 IC50 = 330.0 nM 6.48
CHEMBL603653 IC50 = 400.0 nM 6.40
CHEMBL595157 IC50 = 460.0 nM 6.34
CHEMBL595830 IC50 = 540.0 nM 6.27
CHEMBL604897 IC50 = 580.0 nM 6.24
CHEMBL593526 IC50 = 600.0 nM 6.22
CHEMBL595159 IC50 = 1200.0 nM 5.92
CHEMBL603625 IC50 = 1500.0 nM 5.82
CHEMBL603633 IC50 = 2600.0 nM 5.58
CHEMBL596499 IC50 = 3000.0 nM 5.52
CHEMBL594423 IC50 = 3100.0 nM 5.51
CHEMBL603227 IC50 = 3500.0 nM 5.46
CHEMBL593764 IC50 = 4800.0 nM 5.32
CHEMBL593260 IC50 > 10000.0 nM -