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Assay Detail

CHEMBL1177482

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H] (R)-alpha-methylhistamine from human recombinant histamine H3 receptor expressed in CHO cells after 1 hr by liquid scintillation counting
19
Total Activities
19
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type CHO
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Histamine H3 receptor (CHEMBL264)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis and structure-activity relationships of N-aryl-piperidine derivatives as potent (partial) agonists for human histamine H3 receptor.
Ishikawa M, Furuuchi T, Yamauchi M, Yokoyama F, Kakui N, Sato Y.
Bioorg Med Chem (2010) 18 : 5441 -5448
PubMed 20541426 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 19 8.27 9.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL18661 IMMEPIP 1 9.52
CHEMBL1098230 1 9.37
CHEMBL1172774 1 9.00
CHEMBL1172579 1 9.00
CHEMBL1170855 1 8.85
CHEMBL1169687 1 8.80
CHEMBL1172754 1 8.74
CHEMBL1172775 1 8.27
CHEMBL1173809 1 7.92
CHEMBL1172712 1 7.85
CHEMBL1172776 1 7.85
CHEMBL1173390 1 7.80
CHEMBL1172753 1 7.70
CHEMBL1171443 1 7.52
CHEMBL1173391 1 7.21
CHEMBL1173033 1 6.90
CHEMBL1173032 1 -
CHEMBL1173808 1 -
CHEMBL1173807 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL18661 IMMEPIP Ki = 0.3 nM 9.52
CHEMBL1098230 Ki = 0.43 nM 9.37
CHEMBL1172774 Ki = 1.0 nM 9.00
CHEMBL1172579 Ki = 1.0 nM 9.00
CHEMBL1170855 Ki = 1.4 nM 8.85
CHEMBL1169687 Ki = 1.6 nM 8.80
CHEMBL1172754 Ki = 1.8 nM 8.74
CHEMBL1172775 Ki = 5.4 nM 8.27
CHEMBL1173809 Ki = 12.0 nM 7.92
CHEMBL1172712 Ki = 14.0 nM 7.85
CHEMBL1172776 Ki = 14.0 nM 7.85
CHEMBL1173390 Ki = 16.0 nM 7.80
CHEMBL1172753 Ki = 20.0 nM 7.70
CHEMBL1171443 Ki = 30.0 nM 7.52
CHEMBL1173391 Ki = 61.0 nM 7.21
CHEMBL1173033 Ki = 127.0 nM 6.90
CHEMBL1173032 Ki > 1000.0 nM -
CHEMBL1173808 Ki > 1000.0 nM -
CHEMBL1173807 Ki > 1000.0 nM -