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Assay Detail

CHEMBL1261014

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity to Lymnaea stagnalis His6-AChBP expressed in Bac-to-Bac baculovirus expression system by surface plasmon resonance biosensor assay
22
Total Activities
22
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Lymnaea stagnalis
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Acetylcholine-binding protein (CHEMBL6084)
Type SINGLE PROTEIN
Organism Lymnaea stagnalis

Publication

Surface plasmon resonance biosensor based fragment screening using acetylcholine binding protein identifies ligand efficiency hot spots (LE hot spots) by deconstruction of nicotinic acetylcholine receptor α7 ligands.
de Kloe GE, Retra K, Geitmann M, Källblad P, Nahar T, van Elk R, Smit AB, van Muijlwijk-Koezen JE, Leurs R, Irth H, Danielson UH, de Esch IJ.
J Med Chem (2010) 53 : 7192 -7201
PubMed 20828128 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 22 5.75 7.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1258006 BRADANICLINE 2.0 1 7.70
CHEMBL1258240 1 7.62
CHEMBL1258005 1 7.03
CHEMBL1258126 1 7.00
CHEMBL1257896 1 6.66
CHEMBL1258125 1 6.62
CHEMBL1256001 1 6.50
CHEMBL1258358 1 6.46
CHEMBL1258241 1 6.36
CHEMBL3 NICOTINE 4.0 1 6.32
CHEMBL1256002 1 6.26
CHEMBL1258127 1 6.06
CHEMBL177611 PNU-282987 1 5.44
CHEMBL1255908 1 5.35
CHEMBL1256000 1 5.31
CHEMBL142049 1 5.22
CHEMBL1255909 1 4.73
CHEMBL1255907 1 4.53
CHEMBL1255823 1 4.30
CHEMBL1256003 1 4.13
CHEMBL1256004 1 3.83
CHEMBL1255822 1 3.13

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1258006 BRADANICLINE Kd = 19.95 nM 7.70
CHEMBL1258240 Kd = 23.99 nM 7.62
CHEMBL1258005 Kd = 93.33 nM 7.03
CHEMBL1258126 Kd = 100.0 nM 7.00
CHEMBL1257896 Kd = 218.78 nM 6.66
CHEMBL1258125 Kd = 239.88 nM 6.62
CHEMBL1256001 Kd = 316.23 nM 6.50
CHEMBL1258358 Kd = 346.74 nM 6.46
CHEMBL1258241 Kd = 436.52 nM 6.36
CHEMBL3 NICOTINE Kd = 478.63 nM 6.32
CHEMBL1256002 Kd = 549.54 nM 6.26
CHEMBL1258127 Kd = 870.96 nM 6.06
CHEMBL177611 PNU-282987 Kd = 3630.78 nM 5.44
CHEMBL1255908 Kd = 4466.84 nM 5.35
CHEMBL1256000 Kd = 4897.79 nM 5.31
CHEMBL142049 Kd = 6025.6 nM 5.22
CHEMBL1255909 Kd = 18620.87 nM 4.73
CHEMBL1255907 Kd = 29512.09 nM 4.53
CHEMBL1255823 Kd = 50118.72 nM 4.30
CHEMBL1256003 Kd = 74131.02 nM 4.13
CHEMBL1256004 Kd = 147910.84 nM 3.83
CHEMBL1255822 Kd = 741310.24 nM 3.13