Assay Detail
Functional
CHEMBL1613773
Review assay metadata, readout intent, target linkage, and publication context from the same page.
PUBCHEM_BIOASSAY: Discovery of novel allosteric modulators of the M1 muscarinic receptor: PAM Calcium Assay Dose-Response with M1. Measurement of intracellular Ca2+ using Fluo-4 dye in CHO-K1 cells stably transfected with rat M1 (Class of assay: confirmatory) [Related pubchem assays: 626 (Discovery of Novel Allosteric Modulators of the M1 Muscarinic Receptor: Agonist Primary Screen [Primary Screening])]
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Functional |
| Organism | Rattus norvegicus |
| Category | confirmatory |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Muscarinic acetylcholine receptor M1 (CHEMBL276) ↗| Type | SINGLE PROTEIN |
| Organism | Rattus norvegicus |
Publication
PubChem BioAssay data set
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 11 | 5.65 | 6.07 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL1083203 | — | — | 1 | 6.07 |
| CHEMBL1083204 | — | — | 1 | 6.05 |
| CHEMBL1085686 | — | — | 1 | 5.99 |
| CHEMBL1084940 | — | — | 1 | 5.91 |
| CHEMBL1357815 | — | — | 1 | 5.85 |
| CHEMBL1083205 | — | — | 1 | 5.63 |
| CHEMBL1084941 | — | — | 1 | 5.62 |
| CHEMBL1565295 | — | — | 1 | 5.46 |
| CHEMBL1300017 | — | — | 1 | 5.27 |
| CHEMBL1438248 | — | — | 1 | 5.23 |
| CHEMBL1564753 | — | — | 1 | 5.04 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL1083203 | — | EC50 | = | 845.0 | nM | 6.07 |
| CHEMBL1083204 | — | EC50 | = | 885.0 | nM | 6.05 |
| CHEMBL1085686 | — | EC50 | = | 1030.0 | nM | 5.99 |
| CHEMBL1084940 | — | EC50 | = | 1230.0 | nM | 5.91 |
| CHEMBL1357815 | — | EC50 | = | 1424.0 | nM | 5.85 |
| CHEMBL1083205 | — | EC50 | = | 2320.0 | nM | 5.63 |
| CHEMBL1084941 | — | EC50 | = | 2420.0 | nM | 5.62 |
| CHEMBL1565295 | — | EC50 | = | 3449.0 | nM | 5.46 |
| CHEMBL1300017 | — | EC50 | = | 5324.0 | nM | 5.27 |
| CHEMBL1438248 | — | EC50 | = | 5857.0 | nM | 5.23 |
| CHEMBL1564753 | — | EC50 | = | 9127.0 | nM | 5.04 |